Test Data
(Jurkat cells were treated with 1uM Camptothecin and detected by this reagent and PI.)
Annexin V Conjugate Protein
Annexin V-FITC
Applications
Apoptosis
Synonyms
Annexin V; Annexin V-FITC; Annexin V conjugate protein
Applicable Applications for Annexin V conjugate protein
Apoptosis
Staining Procedure
1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures and centrifuge at 300 g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells, resuspend gently and count the cells.
Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware!
2. Split the cell suspension into tubes, 1-5 × 10^5 cells for each. Centrifuge at 300g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells and discard the supernatant. Add 500 uL of 1 × Annexin V Binding Buffer [#Cat: MBS2557011 ] to resuspend the cells.
3. Add 5 ul of Annexin V-FITC and 5 ul of DNA dye (PI [#Cat: MBS2557012 ] or 7-AAD[#Cat: MBS2557013 ]) to each tube.
4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.
5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.
Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware!
2. Split the cell suspension into tubes, 1-5 × 10^5 cells for each. Centrifuge at 300g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells and discard the supernatant. Add 500 uL of 1 × Annexin V Binding Buffer [#Cat: MBS2557011 ] to resuspend the cells.
3. Add 5 ul of Annexin V-FITC and 5 ul of DNA dye (PI [#Cat: MBS2557012 ] or 7-AAD[#Cat: MBS2557013 ]) to each tube.
4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.
5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.
Preparation and Storage
Store at 4°C for up to one year in dark.
Cautions:
1. For maximal assay performance, this reagent should be used within 3-6 months. Avoid freeze / thaw cycles.
2. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.
3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.
4. For your safety and health, please wear the lab coat and disposable gloves before the experiments.
Cautions:
1. For maximal assay performance, this reagent should be used within 3-6 months. Avoid freeze / thaw cycles.
2. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.
3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.
4. For your safety and health, please wear the lab coat and disposable gloves before the experiments.
Test Data
(Jurkat cells were treated with 1uM Camptothecin and detected by this reagent and PI.)
Test Data
(Jurkat cells were treated with 1uM Camptothecin and detected by this reagent and PI.)
Related Product Information for Annexin V conjugate protein
Annexin V-FITCsdevelopedto identify apoptotic and necrotic cells. Annexin V is a member of the annexin family, which bindsto phosphatidylserine(PS) in a calcium-dependent manner. The fluorescent format of this intracellular protein, Annexin V-FITC, binds specificallyto the PS on outer leaflet of cell membrane by flow cytometry or fluorescence microscopy. Cells at different apoptotic stages canbe distinguished by using Annexin Vand DNA dyes like PropidiumIodide(PI) or 7-AAD.
Product Categories/Family for Annexin V conjugate protein
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Product Notes
The Annexin V (Catalog #AAA2557006) is a Conjugate Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Annexin V can be used in a range of immunoassay formats including, but not limited to, Apoptosis. Researchers should empirically determine the suitability of the Annexin V for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin V, Conjugate Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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