Testing Data
(Jurkat cells were cultured with (Left) or without (Right) 5 ?M Camptothecin for 4 h. Annexin V-ER780 single-positive cells were early apoptotic cells, Annexin V-ER780 and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells were naked nuclei.)
Annexin V-ER780 / DAPI Detection Kit
Annexin V-ER780 / DAPI Apoptosis Detection Kit [A280]
Synonyms
Annexin V-ER780 / DAPI; Annexin V-ER780 / DAPI Apoptosis Detection Kit [A280]; Annexin V-ER780 / DAPI detection kit
Preparation and Storage
Store at 2~8 degree C for one year. Annexin V-ER780 and DAPI Staining Solution should be stored in the dark.
Testing Data
(Jurkat cells were cultured with (Left) or without (Right) 5 ?M Camptothecin for 4 h. Annexin V-ER780 single-positive cells were early apoptotic cells, Annexin V-ER780 and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells were naked nuclei.)
Testing Data
(Jurkat cells were cultured with (Left) or without (Right) 5 ?M Camptothecin for 4 h. Annexin V-ER780 single-positive cells were early apoptotic cells, Annexin V-ER780 and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells were naked nuclei.)
Related Product Information for Annexin V-ER780 / DAPI detection kit
Annexin V-ER780 / DAPI Cell Apoptosis Detection kit is developed to identify apoptotic and necrotic cells.
Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-ER780, the ER780-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.
4',6-Diamidino-2-Phenylindole (DAPI) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to the loss of integrity of membrane, DAPI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and DAPI.
Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-ER780, the ER780-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.
4',6-Diamidino-2-Phenylindole (DAPI) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to the loss of integrity of membrane, DAPI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and DAPI.
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Product Notes
The Annexin V-ER780 / DAPI (Catalog #AAA2567760) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Annexin V-ER780 / DAPI, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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