Flow Cytometry (FC/FACS)
(Staining of CHO-S cells with MBS602640 (filled histogram) or isotype control (open histogram).)
Mouse anti-Human EDG1 Monoclonal Antibody | anti-S1PR1 antibody
EDG1 (Endothelial Cell Differentiation Gene 1) (APC)
Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, 10ul of MBS602640 was added to up to 1x10e6 cells and incubated for 30 minutes at RT.
3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. The cells were resuspended in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2b antibody.
Other applications not tested. Optimal dilutions to be determined by the researcher.
Flow Cytometry (FC/FACS)
(Staining of CHO-S cells with MBS602640 (filled histogram) or isotype control (open histogram).)
Flow Cytometry (FC/FACS)
(Staining of CHO-S cells with MBS602640 (filled histogram) or isotype control (open histogram).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq]
Uniprot Description
EDG-1: a G protein-coupled receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Highly expressed in endothelial cells and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Suggested to be involved in the processes that regulate the migration/differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. S1P-induced endothelial cell migration requires AKT1-mediated phosphorylation of the third intracellular loop. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1
Chromosomal Location of Human Ortholog: 1p21
Cellular Component: integral to membrane; plasma membrane; intrinsic to plasma membrane; endosome; lipid raft; external side of plasma membrane
Molecular Function: G-protein coupled receptor activity; sphingolipid binding; G-protein-coupled receptor binding
Biological Process: regulation of cell adhesion; lamellipodium biogenesis; endothelial cell differentiation; cell migration; regulation of bone resorption; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); negative regulation of stress fiber formation; positive regulation of positive chemotaxis; positive regulation of smooth muscle cell proliferation; transmission of nerve impulse; chemotaxis; blood vessel maturation; neuron differentiation; G-protein coupled receptor protein signaling pathway; actin cytoskeleton reorganization; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; brain development; G-protein signaling, adenylate cyclase inhibiting pathway; cell adhesion; regulation of bone mineralization; positive regulation of cell migration
Research Articles on S1PR1
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Product Notes
The S1PR1 edg1 (Catalog #AAA602640) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EDG1 (Endothelial Cell Differentiation Gene 1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EDG1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Dilution: Flow Cytometry: 10ul labels 10e6 human EDG-1/S1P1-transfected CHO-S cells. Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, 10ul of MBS602640 was added to up to 1x10e6 cells and incubated for 30 minutes at RT. 3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. The cells were resuspended in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2b antibody. Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the S1PR1 edg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EDG1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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