Death Effector Domain Containing Recombinant Protein | DEDD recombinant protein

Recombinant Human Death Effector Domain Containing

Cat. Num. AAA146046

• Gene Names: DEDD; DEFT; KE05; DEDD1; FLDED1; CASP8IP1
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Death Effector Domain Containing; Recombinant Human Death Effector Domain Containing; DEDD Human; Death Effector Domain Containing Human Recombinant; CASP8IP1; DEDD1; DEFT; FLDED1; KE05; DEDPro1; Death effector domain-containing testicular molecule; DEDD recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: DEDD protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MAGLKRRASQ VWPEEHGEQE HGLYSLHRMF DIVGTHLTHR DVRVLSFLFV DVIDDHERGL IRNGRDFLLA LERQGRCDES NFRQVLQLLR IITRHDLLPY VTLKRRRAVC PDLVDKYLEE TSIRYVTPRA LSDPEPRPPQ PSKTVPPHYP VVCCPTSGPQ MCSKRPARGR ATLGSQRKRR KSVTPDPKEK QTCDIRLRVR AEYCQHETAL QGNVFSNKQD PLERQFERFN QANTILKSRD LGSIICDIKF SELTYLDAFW RDYINGSLLE ALKGVFITDS LKQAVGHEAI KLLVNVDEED YELGRQKLLR NLMLQALP
• Sequence Length: 318

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for DEDD recombinant protein

Description: DEDD Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 338 amino acids (1-318a.a) and having a molecular mass of 38.9kDa. DEDD is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Death effector domain-containing protein (DEDD) is a cytoplasmic protein. The cell death activity of DEDD relates to its nuclear localization. DEDD translocates to the nucleus during CD95-mediated apoptosis, there it localizes to nucleoli-like structures, activates caspase-6 and particularly inhibits RNA polymerase I-dependent transcription. DEDD is usually expressed in a variety of tissues, and found in the highest levels in the testis. Overexpression of DEDD was shown to induce weak apoptosis.

Product Categories/Family for DEDD recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 89353285
• NCBI GeneID: 9191
• NCBI Accession #: NP_001034800.1
• NCBI GenBank Nucleotide #: NM_001039711.1
• UniProt Accession #: O75618; O60737; D3DVF5
• Molecular Weight: 40,418 Da
• NCBI Official Full Name: death effector domain-containing protein
• NCBI Official Synonym Full Names: death effector domain containing
• NCBI Official Symbol: DEDD
• NCBI Official Synonym Symbols: DEFT; KE05; DEDD1; FLDED1; CASP8IP1
• NCBI Protein Information: death effector domain-containing protein; DEDPro1; FLDED-1; death effector domain-containing testicular molecule
• UniProt Protein Name: Death effector domain-containing protein
• Protein Family: Death effector domain-containing protein
• UniProt Gene Name: DEDD
• UniProt Synonym Gene Names: DEDPRO1; DEFT
• UniProt Entry Name: DEDD_HUMAN

NCBI Description

This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DEDD: A scaffold protein that directs CASP3 to certain substrates and facilitates their ordered degradation during apoptosis. May also play a role in mediating CASP3 cleavage of KRT18. Regulates degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. Inhibits DNA transcription in vitro. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: cytoplasm; nucleolus

Molecular Function: protein binding; DNA binding

Biological Process: regulation of apoptosis; induction of apoptosis via death domain receptors; transcription, DNA-dependent; spermatogenesis; decidualization; negative regulation of protein catabolic process