Typical Testing Data/Standard Curve (for reference only)
Human Virus Induced Signaling Adapter (VISA) CLIA Kit | VISA clia kit
Virus Induced Signaling Adapter (VISA) CLIA Kit
Gene Names
MAVS; IPS1; VISA; IPS-1; CARDIF
Reactivity
Human
Applications
Chemiluminescent Immunoassay (CLIA)
Synonyms
Virus Induced Signaling Adapter (VISA); Virus Induced Signaling Adapter (VISA) CLIA Kit; CARDIF; IPS1; MAVS; Mitochondrial Antiviral-Signaling Protein; Interferon beta promoter stimulator protein 1; CARD Adaptor Inducing IFN-beta; NF-kappa-B-activating protein 031N; VISA clia kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of VISA. No significant cross-reactivity or interference between VISA and analogues was observed.
Applicable Applications for VISA clia kit
Chemiluminescent Immunoassay (CLIA)
Application Notes
Chemiluminescent immunoassay for Antigen Detection.
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.060ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VISA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VISA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for VISA clia kit
Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of VISA in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to VISA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VISA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the VISA level in the sample or standard.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to VISA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VISA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the VISA level in the sample or standard.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
540
NCBI Official Full Name
mitochondrial antiviral-signaling protein isoform 2
NCBI Official Synonym Full Names
mitochondrial antiviral signaling protein
NCBI Official Symbol
MAVS
NCBI Official Synonym Symbols
IPS1; VISA; IPS-1; CARDIF
NCBI Protein Information
mitochondrial antiviral-signaling protein; IFN-B promoter stimulator 1; CARD adaptor inducing IFN-beta; virus-induced signaling adaptor; virus-induced-signaling adapter; CARD adapter inducing interferon beta; putative NF-kappa-B-activating protein 031N; i
UniProt Protein Name
Mitochondrial antiviral-signaling protein
UniProt Gene Name
MAVS
UniProt Synonym Gene Names
IPS1; KIAA1271; VISA; MAVS; Cardif; IPS-1; VISA
UniProt Entry Name
MAVS_HUMAN
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Product Notes
The Human VISA mavs (Catalog #AAA2709436) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709436 CLIA Kit recognizes Human VISA. AAA Biotech's Virus Induced Signaling Adapter (VISA) can be used in a range of immunoassay formats including, but not limited to, Chemiluminescent Immunoassay (CLIA). Chemiluminescent immunoassay for Antigen Detection. Researchers should empirically determine the suitability of the VISA mavs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Virus Induced Signaling Adapter (VISA), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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