DEDD recombinant protein

DEDD, 1-318aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205493

• Gene Names: DEDD; DEFT; KE05; DEDD1; FLDED1; CASP8IP1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: DEDD; 1-318aa; Human; His tag; E Coli (Denatured); Death effector domain-containing protein; CASP8IP1; DEDD1; DEFT; FLDED1; KE05; DEDD recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAGLKRRASQ VWPEEHGEQE HGLYSLHRMF DIVGTHLTHR DVRVLSFLFV DVIDDHERGL IRNGRDFLLA LERQGRCDES NFRQVLQLLR IITRHDLLPY VTLKRRRAVC PDLVDKYLEE TSIRYVTPRA LSDPEPRPPQ PSKTVPPHYP VVCCPTSGPQ MCSKRPARGR ATLGSQRKRR KSVTPDPKEK QTCDIRLRVR AEYCQHETAL QGNVFSNKQD PLERQFERFN QANTILKSRD LGSIICDIKF SELTYLDAFW RDYINGSLLE ALKGVFITDS LKQAVGHEAI KLLVNVDEED YELGRQKLLR NLMLQALP
• Sequence Length: 318

• Applicable Applications for DEDD recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DEDD recombinant protein

Death effector domain-containing protein, also known as DEDD, is a cytoplasmic protein. It translocates to the nucleus during CD95-mediated apoptosis, where it localizes to nucleoli-like structures, activates caspase-6 and specifically inhibits RNA polymerase I-dependent transcription. The cell death activity of DEDD relates to its nuclear localization. DEDD is widely expressed in a variety of tissues, with highest levels in the testis. Overexpression of this gene was shown to induce weak apoptosis. Recombinant human DEDD protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for DEDD recombinant protein

Apoptosis

References

Stegh A H., et al. (1998) EMBO J. 17:5974-5986.; Schickling O., et al. (2001) Cell Death Differ. 8:1157-1168.

NCBI and Uniprot Product Information

• NCBI GI #: 14670394
• NCBI GeneID: 9191
• NCBI Accession #: NP_127491
• NCBI GenBank Nucleotide #: NM_032998.2
• UniProt Accession #: O75618; O60737; D3DVF5
• Molecular Weight: 38.9 kDa (338aa)
• NCBI Official Full Name: death effector domain-containing protein isoform b
• NCBI Official Synonym Full Names: death effector domain containing
• NCBI Official Symbol: DEDD
• NCBI Official Synonym Symbols: DEFT; KE05; DEDD1; FLDED1; CASP8IP1
• NCBI Protein Information: death effector domain-containing protein
• UniProt Protein Name: Death effector domain-containing protein
• Protein Family: Death effector domain-containing protein
• UniProt Gene Name: DEDD
• UniProt Synonym Gene Names: DEDPRO1; DEFT

NCBI Description

This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

A scaffold protein that directs CASP3 to certain substrates and facilitates their ordered degradation during apoptosis. May also play a role in mediating CASP3 cleavage of KRT18. Regulates degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. Inhibits DNA transcription in vitro ().