Rabbit BAT3/BAG-6 Monoclonal Antibody | anti-BAG6 antibody

BAT3/BAG-6 Rabbit mAb

Cat. Num. AAA4752611

• Gene Names: BAG6; G3; BAT3; BAG-6; D6S52E
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Affinity purified
• Synonyms: BAT3/BAG-6; Monoclonal Antibody; BAT3/BAG-6 Rabbit mAb; Large proline-rich protein BAG6; G3; BAT3; BAG-6; D6S52E; anti-BAG6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R04-1C1
• Specificity: Expressed by immature dendritic cells (at protein level).
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-BAG6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:20-1:50; ICC/IF: 1:50; FC/FACS: 1:20
• Immunogen: A synthetic peptide of human BAT3
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cytoplasm, Nucleus, Secreted
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of BAT3/BAG-6 in K562,Rat Brain,C6,3T3 cell lysates using BAT3/BAG-6 Rabbit mAb(1:1000 diluted). Predicted band size:119kDa. Observed band size:150kDa.)

Related Product Information for anti-BAG6 antibody

This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
ATP-independent molecular chaperone preventing the aggregation of misfolded and hydrophobic patches-containing proteins (PubMed:21636303). Functions as part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, which maintains these client proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20516149, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20516149, PubMed:20676083, PubMed:28104892, PubMed:25535373). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated by RNF126, an E3 ubiquitin-protein ligase associated with BAG6 and are sorted to the proteasome (PubMed:24981174, PubMed:28104892, PubMed:27193484). SGTA which prevents the recruitment of RNF126 to BAG6 may negatively regulate the ubiquitination and the proteasomal degradation of client proteins (PubMed:23129660, PubMed:25179605, PubMed:27193484). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303). BAG6 is also required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, it may participate to the production of antigenic peptides and play a role in antigen presentation in immune response. BAG6 is also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. BAG6 may ensure the proper degradation of these proteins and thereby protects the endoplasmic reticulum from protein overload upon stress (PubMed:26565908). By inhibiting the polyubiquitination and subsequent proteasomal degradation of HSPA2 it may also play a role in the assembly of the synaptonemal complex during spermatogenesis. Also positively regulates apoptosis by interacting with and stabilizing the proapoptotic factor AIFM1. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (PubMed:26692333).

NCBI and Uniprot Product Information

• NCBI GI #: 149158692
• NCBI GeneID: 7917
• NCBI Accession #: NP_004630.3
• NCBI GenBank Nucleotide #: NM_004639.3
• UniProt Accession #: P46379; O95874; A2ADJ7; A3KQ42; A3KQ44; A6NGY6; A6PWF7; B0UX84; B4DZ12; B4E3V4; E7EMZ4; F8VXY4
• Molecular Weight: 113,456 Da
• NCBI Official Full Name: large proline-rich protein BAG6 isoform a
• NCBI Official Synonym Full Names: BCL2-associated athanogene 6
• NCBI Official Symbol: BAG6
• NCBI Official Synonym Symbols: G3; BAT3; BAG-6; D6S52E
• NCBI Protein Information: large proline-rich protein BAG6; scythe; protein G3; protein Scythe; HLA-B associated transcript 3; HLA-B associated transcript-3; HLA-B-associated transcript 3; large proline-rich protein BAT3; BAG family molecular chaperone regulator 6
• UniProt Protein Name: Large proline-rich protein BAG6
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG6
• UniProt Synonym Gene Names: BAT3; G3; BAG-6; BAG6
• UniProt Entry Name: BAG6_HUMAN

NCBI Description

This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BAT3: Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain. Interacts with L.pneumophila proteins Lpg2160 and LegU1. Interacts with NCR3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Apoptosis; Cell cycle regulation; Chaperone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding; ribosome binding; Hsp70 protein binding; polyubiquitin binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of proteolysis; synaptonemal complex assembly; internal peptidyl-lysine acetylation; protein stabilization; immune system process; chromatin modification; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of cell proliferation; embryonic development; transport; spermatogenesis; brain development; kidney development; cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; lung development; negative regulation of apoptosis