Human TGF-beta2 ELISA Kit | TGFB2 elisa kit

TGF-beta2 (Human) OmniKine ELISA Kit

Cat. Num. AAA9501989

• Gene Names: TGFB2; LDS4; G-TSF; TGF-beta2
• Reactivity: Human
• Synonyms: TGF-beta2; TGF-beta2 (Human) OmniKine ELISA Kit; Transforming growth factor beta-2; BSC-1 cell growth inhibitor; Cetermin; Glioblastoma-derived T-cell suppressor factor; Polyergin; TGFB2 elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human TGF-beta2 ELISA is capable of recognizing both recombinant and naturally produced Human TGF-beta2 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 442

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human TGF-beta2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human TGF-beta2 proteins within the range of 32-2000 pg/ml.)

Related Product Information for TGFB2 elisa kit

Principle of the Assay: The OmniKine™ Human TGF-beta2 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human TGF-beta2 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human TGF-beta2 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t (1; 7) (q41; p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 208022653
• NCBI GeneID: 7042
• NCBI Accession #: NP_001129071.1
• NCBI GenBank Nucleotide #: NM_001135599.3
• UniProt Accession #: P61812; P08112; Q15579; Q15581; Q4VAV9; B4DKC5
• Molecular Weight: 50,573 Da
• NCBI Official Full Name: transforming growth factor beta-2 isoform 1
• NCBI Official Synonym Full Names: transforming growth factor beta 2
• NCBI Official Symbol: TGFB2
• NCBI Official Synonym Symbols: LDS4; G-TSF; TGF-beta2
• NCBI Protein Information: transforming growth factor beta-2
• UniProt Protein Name: Transforming growth factor beta-2
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFB2
• UniProt Synonym Gene Names: TGF-beta-2; G-TSF; LAP

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers. A chromosomal translocation that includes this gene is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. Mutations in this gene may be associated with Loeys-Dietz syndrome. This gene encodes multiple isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]

Uniprot Description

TGFB2: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Homodimer; disulfide-linked. Heterodimers with TGFB1 and with TGFB3 have been found in bone. Interacts with the serine proteases, HTRA1 and HTRA3. Interacts with ASPN. Belongs to the TGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: axon; cell soma; extracellular matrix; extracellular region; extracellular space

Molecular Function: amyloid-beta binding; cytokine activity; growth factor activity; protein binding; protein heterodimerization activity; protein homodimerization activity; receptor binding; transforming growth factor beta receptor binding; type II transforming growth factor beta receptor binding

Biological Process: activation of protein kinase activity; angiogenesis; BMP signaling pathway; cardiac muscle cell proliferation; cardioblast differentiation; catagen; cell cycle arrest; cell death; cell development; cell growth; cell migration; cell morphogenesis; cell proliferation; cell-cell junction organization; cell-cell signaling; collagen fibril organization; dopamine biosynthetic process; embryonic development; embryonic gut development; embryonic limb morphogenesis; epithelial to mesenchymal transition; eye development; generation of neurons; glial cell migration; hair follicle development; hair follicle morphogenesis; heart development; heart morphogenesis; hemopoiesis; inner ear development; kidney development; male gonad development; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of immune response; negative regulation of Ras protein signal transduction; neural tube closure; neuron development; neutrophil chemotaxis; odontogenesis; palate development; platelet degranulation; positive regulation of cardioblast differentiation; positive regulation of catagen; positive regulation of cell adhesion mediated by integrin; positive regulation of cell cycle; positive regulation of cell division; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of heart contraction; positive regulation of immune response; positive regulation of integrin biosynthetic process; positive regulation of neuron apoptotic process; positive regulation of ossification; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein secretion; positive regulation of stress-activated MAPK cascade; protein amino acid phosphorylation; regulation of transforming growth factor-beta2 production; response to drug; response to hypoxia; response to progesterone; response to wounding; salivary gland morphogenesis; skeletal system development; SMAD protein import into nucleus; somatic stem cell division; transforming growth factor beta receptor signaling pathway; uterine wall breakdown; uterus development; wound healing

Disease: Loeys-dietz Syndrome 4