DECR1 recombinant protein

DECR1, 35-335aa, Human, His tag, E Coli

Cat. Num. AAA203975

• Gene Names: DECR1; DECR; NADPH; SDR18C1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: DECR1; 35-335aa; Human; His tag; E Coli; 24-dienoyl-CoA reductase mitochondrial; 2; 4-dienoyl-CoA reductase; mitochondrial; DECR; NADPH; SDR18C1; DECR1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MNTEALQSKF FSPLQKAMLP PNSFQGKVAF ITGGGTGLGK GMTTLLSSLG AQCVIASRKM DVLKATAEQI SSQTGNKVHA IQCDVRDPDM VQNTVSELIK VAGHPNIVIN NAAGNFISPT ERLSPNAWKT ITDIVLNGTA FVTLEIGKQL IKAQKGAAFL SITTIYAETG SGFVVPSASA KAGVEAMSKS LAAEWGKYGM RFNVIQPGPI KTKGAFSRLD PTGTFEKEMI GRIPCGRLGT VEELANLAAF LCSDYASWIN GAVIKFDGGE EVLISGEFND LRKVTKEQWD TIEELIRKTK GS
• Sequence Length: 335

• Applicable Applications for DECR1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DECR1 recombinant protein

DECR1 is a mitochondrial protein that exists as a homotetramer and belongs to the family of short-chain dehydrogenases/reductases. It functions as an auxiliary enzyme of beta-oxidation andt participates in the metabolism of unsaturated fatty enoyl-CoA esters. Specifically, DECR1 uses NADP+ to catalyze the reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA, which can then be used as an intermediate in the Krebs cycle. Additionally, DECR1 is thought to function as a tumor suppressor, possibly downregulating the expression of Neu and slowing the rate of tumorigenesis. Recombinant human DECR1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for DECR1 recombinant protein

Cancer

References

Koivuranta KT., et al. (1994) Biochem J. 304 (3):787-92. ; Roe CR., et al. (1990) J Clin Invest. 85 (5):1703-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4503301
• NCBI GeneID: 1666
• NCBI Accession #: NP_001350
• NCBI GenBank Nucleotide #: NM_001359.1
• UniProt Accession #: Q16698; Q2M304; Q93085; B7Z6B8
• Molecular Weight: 34.4 kDa (322aa), confirmed by MALDI-TOF
• NCBI Official Full Name: 2,4-dienoyl-CoA reductase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: 2,4-dienoyl-CoA reductase 1
• NCBI Official Symbol: DECR1
• NCBI Official Synonym Symbols: DECR; NADPH; SDR18C1
• NCBI Protein Information: 2,4-dienoyl-CoA reductase, mitochondrial
• UniProt Protein Name: 2,4-dienoyl-CoA reductase, mitochondrial
• Protein Family: 2,4-dienoyl-CoA reductase
• UniProt Gene Name: DECR1
• UniProt Synonym Gene Names: DECR; SDR18C1; 4-enoyl-CoA reductase [NADPH]

NCBI Description

This gene encodes an accessory enzyme which participates in the beta-oxidation and metabolism of unsaturated fatty enoyl-CoA esters. [provided by RefSeq, Jul 2008]

Uniprot Description

Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.