Rabbit anti-Human, Mouse BBC3 Polyclonal Antibody | anti-BBC3 antibody

BBC3 Antibody

Cat. Num. AAA9402439

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Synonyms: BBC3; Polyclonal Antibody; BBC3 Antibody; Bcl-2-binding component 3; JFY1; PUMA; JFY-1; anti-BBC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total BBC3 protein.
• Form/Format: Supplied at 1.1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 193

• Applicable Applications for anti-BBC3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000-1:5000; Western blotting: 1:500-1:2000
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from 180-193 amino acids of Human Bcl-2-binding component 3
• Target Name: BBC3
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 8+10%SDS-PAGELane1: NIH/3T3 cell lysateLane2: A549 cell lysateLane3: Hela cell lysateLysates: 30ug per lanePrimary antibody: 1/540 dilutionSecondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/5000 dilutionExposure time: 1 minute)

Related Product Information for anti-BBC3 antibody

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-BBC3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 15193488
• NCBI GeneID: 27113
• NCBI Accession #: NP_055232.1
• NCBI GenBank Nucleotide #: NM_014417.4
• Molecular Weight: 10,326 Da
• NCBI Official Full Name: bcl-2-binding component 3 isoform 4
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3
• UniProt Protein Name: Bcl-2-binding component 3
• Protein Family: Bcl-2-binding component
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; release of sequestered calcium ion into cytosol; positive regulation of neuron apoptosis; reduction of endoplasmic reticulum calcium ion concentration; determination of adult life span; response to DNA damage stimulus; negative regulation of growth