Immunohistochemistry (IHC)
((0.3ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
anti-Mouse Aspartyl-tRNA Synthetase Antibody | anti-Dars antibody
Goat anti-Aspartyl-tRNA Synthetase (N terminus) Antibody
Gene Names
Dars; 5730439G15Rik
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Aspartyl-tRNA Synthetase; Goat anti-Aspartyl-tRNA Synthetase (N terminus) Antibody; Dars; aspartyl-tRNA synthetase; 5730439G15Rik; aspRS; cytoplasmic; anti-Dars antibody
Reactivity
Mouse
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
501
Applicable Applications for anti-Dars antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:128000.
WB: Approx 55kDa band observed in Mouse fetal Brain lysates. Recommended concentration: 0.3-1ug/ml.
WB: Approx 55kDa band observed in Mouse fetal Brain lysates. Recommended concentration: 0.3-1ug/ml.
Immunogen Sequence
Peptide with sequence SRKGQEKPREIVD-C, from the N Terminus of the protein sequence according to NP_803228.2; NP_663482.2.
Peptide Sequence + C
SRKGQEKPREIVD-C
Epitope
N Terminus
Note
This antibody is expected to recognize reported isoform 1 (NP_803228.2) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
((0.3ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((0.3ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57.1kDa
NCBI Official Full Name
aspartate--tRNA ligase, cytoplasmic isoform 1
NCBI Official Synonym Full Names
aspartyl-tRNA synthetase
NCBI Official Symbol
Dars
NCBI Official Synonym Symbols
5730439G15Rik
NCBI Protein Information
aspartate--tRNA ligase, cytoplasmic
UniProt Protein Name
Aspartate--tRNA ligase, cytoplasmic
UniProt Gene Name
Dars
UniProt Synonym Gene Names
AspRS
Uniprot Description
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Research Articles on Dars
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Product Notes
The Dars dars (Catalog #AAA423801) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Aspartyl-tRNA Synthetase (N terminus) Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartyl-tRNA Synthetase can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:128000. WB: Approx 55kDa band observed in Mouse fetal Brain lysates. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the Dars dars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartyl-tRNA Synthetase, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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