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Western Blot (WB)
(Anti-Hspa5 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:72 kDa)
Rabbit anti-Mouse, Human GRP78/HSPA5 Polyclonal Antibody | anti-HSPA5 antibody
Anti-GRP78/HSPA5 Antibody, Rabbit Polyclonal
Gene Names
Hspa5; Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; AL022860; AU019543; D2Wsu17e; D2Wsu141e
Reactivity
Mouse, Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
GRP78/HSPA5; Polyclonal Antibody; Anti-GRP78/HSPA5 Antibody; Rabbit Polyclonal; GRP78/Hspa5 Antibody; Rabbit PAb; Antigen Affinity Purified; heat shock 70kDa protein 5 (glucose-regulated protein; 78kDa); Anti-AL022860 Antibody; Anti-AU019543 Antibody; Anti-baffled Antibody; Anti-Bip Antibody; Anti-D2Wsu141e Antibody; Anti-D2Wsu17e Antibody; Anti-Grp78 Antibody; Anti-Hsce70 Antibody; Anti-mBiP Antibody; Anti-SEZ-7 Antibody; Anti-Sez7 Antibody; anti-HSPA5 antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse GRP78/HSPA5
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-HSPA5 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:2500-1:10,000
IHC-P: 1:2500-1:10,000
Immunogen
A synthetic peptide corresponding to the N-terminus of the Mouse GRP78/Hspa5
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse GRP78/Hspa5, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-Hspa5 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:72 kDa)
Western Blot (WB)
(Anti-Hspa5 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:72 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining Hspa5 in mouse brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining Hspa5 in mouse brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining Hspa5 in mouse liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining Hspa5 in mouse liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining Hspa5 in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining Hspa5 in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining Hspa5 in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining Hspa5 in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
72,422 Da
NCBI Official Full Name
78 kDa glucose-regulated protein
NCBI Official Synonym Full Names
heat shock protein 5
NCBI Official Symbol
Hspa5
NCBI Official Synonym Symbols
Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; AL022860; AU019543; D2Wsu17e; D2Wsu141e
NCBI Protein Information
78 kDa glucose-regulated protein
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Gene Name
Hspa5
UniProt Synonym Gene Names
BiP
Uniprot Description
Plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum (PubMed:12475965). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate (PubMed:12411443).
Research Articles on HSPA5
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Product Notes
The HSPA5 hspa5 (Catalog #AAA8102684) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GRP78/HSPA5 Antibody, Rabbit Polyclonal reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's GRP78/HSPA5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 IHC-P: 1:2500-1:10,000. Researchers should empirically determine the suitability of the HSPA5 hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP78/HSPA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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