Immunohistochemistry (IHC)
((1ug/ml) staining of Human Lymph Node (A) and (0.5ug/ml) Tonsil (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
anti-Human LAG3/CD223 Antibody | anti-LAG3 antibody
Goat anti-LAG3/CD223 Antibody
Gene Names
LAG3; CD223
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
LAG3/CD223; Goat anti-LAG3/CD223 Antibody; Goat Anti-LAG3 Antibody; LAG3; lymphocyte activating 3; CD223; lymphocyte-activation gene 3; anti-LAG3 antibody
Reactivity
Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
525
Applicable Applications for anti-LAG3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:2000.
WB: Approx 55kDa band observed in Human Lymph Node and Tonsil lysates. Recommended concentration: 0.3-1ug/ml.
WB: Approx 55kDa band observed in Human Lymph Node and Tonsil lysates. Recommended concentration: 0.3-1ug/ml.
Immunogen Sequence
Peptide with sequence EDVSQAQAGTYTCH, from the internal region of the protein sequence according to NP_002277.4.
Peptide Sequence + C
EDVSQAQAGTYTCH
Epitope
Internal Region
Note
This antibody is expected to recognize the extra-cellular domain.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
((1ug/ml) staining of Human Lymph Node (A) and (0.5ug/ml) Tonsil (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((1ug/ml) staining of Human Lymph Node (A) and (0.5ug/ml) Tonsil (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57.4kDa
NCBI Official Full Name
lymphocyte activation gene 3 protein
NCBI Official Synonym Full Names
lymphocyte activating 3
NCBI Official Symbol
LAG3
NCBI Official Synonym Symbols
CD223
NCBI Protein Information
lymphocyte activation gene 3 protein
UniProt Protein Name
Lymphocyte activation gene 3 protein
Protein Family
UniProt Gene Name
LAG3
UniProt Synonym Gene Names
FDC; LAG-3
NCBI Description
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
Uniprot Description
Involved in lymphocyte activation. Binds to HLA class-II antigens.
Research Articles on LAG3
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Product Notes
The LAG3 lag3 (Catalog #AAA423823) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LAG3/CD223 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAG3/CD223 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:2000. WB: Approx 55kDa band observed in Human Lymph Node and Tonsil lysates. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the LAG3 lag3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAG3/CD223, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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