Typical Testing Data/Standard Curve (for reference only)
Rat chemokine (C-X-C motif) ligand 2 ELISA Kit | CXCL2 elisa kit
Rat macrophage inflammatory protein 2, MIP-2 ELISA Kit
Gene Names
Cxcl2; Mip-2; Scyb2
Reactivity
Rat
Synonyms
chemokine (C-X-C motif) ligand 2; Rat macrophage inflammatory protein 2; MIP-2 ELISA Kit; MIP-2 ELISA kit; CINC-2a; GRO2; GROb; MGSA-b; MIP-2a; MIP2; MIP2A; SCYB2; GRO2 oncogene; MGSA beta; melanoma growth stimulatory activity beta; CXCL2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat MIP-2. No significant cross-reactivity or interference between rat MIP-2 and analogues was observed.
Sequence Length
100
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
6.25 pg/ml -400 pg/ml.
Sensitivity
The minimum detectable dose of rat MIP-2 is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCL2 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,783 Da
NCBI Official Full Name
C-X-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 2
NCBI Official Symbol
Cxcl2
NCBI Official Synonym Symbols
Mip-2; Scyb2
NCBI Protein Information
C-X-C motif chemokine 2; MIP2; CINC-3; macrophage inflammatory protein 2; small inducible cytokine subfamily, member 2; cytokine-induced neutrophil chemoattractant 3
UniProt Protein Name
C-X-C motif chemokine 2
Protein Family
UniProt Gene Name
Cxcl2
UniProt Synonym Gene Names
Cinc3; Mip-2; Mip2; Scyb2; CINC-3; MIP2
UniProt Entry Name
CXCL2_RAT
NCBI Description
chemokine involved in the pulmonary inflammatory response [RGD, Feb 2006]
Uniprot Description
Function: Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
Research Articles on CXCL2
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Product Notes
The Rat CXCL2 cxcl2 (Catalog #AAA700890) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700890 ELISA Kit recognizes Rat CXCL2. It is sometimes possible for the material contained within the vial of "chemokine (C-X-C motif) ligand 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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