Typical Testing Data/Standard Curve (for reference only)
Mouse hepatocyte growth factor (hepapoietin A; scatter factor) ELISA Kit | HGF elisa kit
Mouse hepatocyte growth factor, HGF ELISA Kit
Gene Names
Hgf; SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
Reactivity
Mouse
Synonyms
hepatocyte growth factor (hepapoietin A; scatter factor); Mouse hepatocyte growth factor; HGF ELISA Kit; HGF ELISA kit; DFNB39; F-TCF; HGFB; HPTA; SF; OTTHUMP00000206710; fibroblast-derived tumor cytotoxic factor; hepatocyte growth factor; lung fibroblast-derived mitogen; HGF elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse HGF. No significant cross-reactivity or interference between mouse HGF and analogues was observed.
Sequence Length
728
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 pg/ml -1000 pg/ml.
Sensitivity
The minimum detectable dose of mouse HGF is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HGF elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,945 Da
NCBI Official Full Name
hepatocyte growth factor isoform 1 preproprotein
NCBI Official Synonym Full Names
hepatocyte growth factor
NCBI Official Symbol
Hgf
NCBI Official Synonym Symbols
SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
NCBI Protein Information
hepatocyte growth factor; scatter factor; hepatopoietin-A
UniProt Protein Name
Hepatocyte growth factor
Protein Family
UniProt Gene Name
Hgf
UniProt Synonym Gene Names
SF
UniProt Entry Name
HGF_MOUSE
Uniprot Description
Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.1 PublicationManual assertion based on experiment in:Ref.13
Research Articles on HGF
1. Differences in the intensity and quality of melanin biosynthesis as a result of HGF overexpression.
Similar Products
Product Notes
The Mouse HGF hgf (Catalog #AAA701111) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701111 ELISA Kit recognizes Mouse HGF. It is sometimes possible for the material contained within the vial of "hepatocyte growth factor (hepapoietin A; scatter factor), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
