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Typical Testing Data/Standard Curve (for reference only)

Human chemokine (C-X-C motif) ligand 3 ELISA Kit | CXCL3 elisa kit

Human C-X-C motif chemokine 3, CXCL3 ELISA Kit

Gene Names
CXCL3; GRO3; GROg; MIP2B; SCYB3; MIP-2b; CINC-2b
Reactivity
Human
Synonyms
chemokine (C-X-C motif) ligand 3; Human C-X-C motif chemokine 3; CXCL3 ELISA Kit; Human C-X-C motif chemokine 3 (CXCL3) ELISA kit; CINC-2b; GRO3; GROg; MIP-2b; MIP2B; SCYB3; GRO3 oncogene; MGSA gamma; melanoma growth stimulatory activity gamma; CXCL3 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human CXCL3. No significant cross-reactivity or interference between human CXCL3 and analogues was observed.
Sequence Length
107
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
9.37 pg/ml -600 pg/ml.
Sensitivity
The minimum detectable dose of human CXCL3 is typically less than 2.34 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCL3 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CXCL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
References

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,342 Da
NCBI Official Full Name
C-X-C motif chemokine 3
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 3
NCBI Official Symbol
CXCL3
NCBI Official Synonym Symbols
GRO3; GROg; MIP2B; SCYB3; MIP-2b; CINC-2b
NCBI Protein Information
C-X-C motif chemokine 3; GRO-gamma; MIP2-beta; MGSA gamma; GRO3 oncogene; GRO-gamma(1-73); growth-regulated protein gamma; macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma
UniProt Protein Name
C-X-C motif chemokine 3
Protein Family
UniProt Gene Name
CXCL3
UniProt Synonym Gene Names
GRO3; GROG; SCYB3; GRO-gamma; MIP2-beta
UniProt Entry Name
CXCL3_HUMAN

NCBI Description

This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; immune response; inflammatory response; regulation of cell proliferation

Research Articles on CXCL3

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Product Notes

The Human CXCL3 cxcl3 (Catalog #AAA910472) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA910472 ELISA Kit recognizes Human CXCL3. It is sometimes possible for the material contained within the vial of "chemokine (C-X-C motif) ligand 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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