Mouse chemokine (C-X3-C motif) ligand 1 ELISA Kit | CX3CL1 elisa kit

Mouse Fractalkine, FK ELISA Kit

Cat. Num. AAA703369

• Gene Names: Cx3cl1; CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e
• Reactivity: Mouse
• Synonyms: chemokine (C-X3-C motif) ligand 1; Mouse Fractalkine; FK ELISA Kit; ABCD-3; C3Xkine; CXC3; CXC3C; NTN; NTT; SCYD1; fractalkine; neurotactin; small inducible cytokine subfamily D (Cys-X3-Cys); member 1 (fractalkine; neurotactin) ; small inducible cytokine subfamily D (; CX3CL1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse CX3CL1. No significant cross-reactivity or interference between Mouse CX3CL1 and analogues was observed.
• Sequence Length: 395

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 125 pg/ml -8000 pg/ml
• Sensitivity: 31.25 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cell Adhesion
• Protein Biological Process 3: Cell adhesion
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CX3CL1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fkn. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Fkn and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Fkn, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Fkn in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31982017
• NCBI GeneID: 20312
• NCBI Accession #: NP_033168.2
• NCBI GenBank Nucleotide #: NM_009142.3
• UniProt Accession #: O35188; O35933; Q91V44
• Molecular Weight: 42,099 Da
• NCBI Official Full Name: fractalkine
• NCBI Official Synonym Full Names: chemokine (C-X3-C motif) ligand 1
• NCBI Official Symbol: Cx3cl1
• NCBI Official Synonym Symbols: CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e
• NCBI Protein Information: fractalkine; neurotactin; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D, 1
• UniProt Protein Name: Fractalkine
• Protein Family: Fractalkine
• UniProt Gene Name: Cx3cl1
• UniProt Synonym Gene Names: Cx3c; Fkn; Scyd1
• UniProt Entry Name: X3CL1_MOUSE

Uniprot Description

Function: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium Binds to CX3CR1.