Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) Recombinant Protein | ENPP1 recombinant protein

Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1)

Cat. Num. AAA2009087

• Gene Names: ENPP1; M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1); Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1); ENPP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- DERPHFYTL YLEEPDSSGH SYGPVSSEVI KALQRVDGMV GMLMDGLKEL NLHRCLNLIL ISDHGMEQGS CKKYIYLNKY LGDVKNIKVI YGPAARLRPS DVPDKYYSFN YEGIARNLSC REPNQHFKPY LKHFLPKRLH FAKSDRIEPL TFYLDPQW
• Sequence Length: 925

• Applicable Applications for ENPP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asp362~Trp518 (Accession # P22413) with N-terminal His-Tag
• Subcellular Location: Membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 7.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 170650661
• NCBI GeneID: 5167
• NCBI Accession #: NP_006199.2
• NCBI GenBank Nucleotide #: NM_006208.2
• UniProt Accession #: P22413; Q5T9R6; Q9NPZ3; Q9P1P6; Q9UP61; Q9Y6K3
• Molecular Weight: 19.8kDa
• NCBI Official Full Name: ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 1
• NCBI Official Symbol: ENPP1
• NCBI Official Synonym Symbols: M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Ly-41 antigen; alkaline phosphodiesterase 1; plasma-cell membrane glycoprotein 1; plasma-cell membrane glycoprotein PC-1; membrane component chromosome 6 surface marker 1; phosphodiesterase I/nucleotide pyrophosphatase 1; membrane component, chromosome 6, surface marker 1
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• Protein Family: Ectonucleotide pyrophosphatase/phosphodiesterase family
• UniProt Gene Name: ENPP1
• UniProt Synonym Gene Names: M6S1; NPPS; PC1; PDNP1; E-NPP 1; NPPase
• UniProt Entry Name: ENPP1_HUMAN

NCBI Description

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Motility/polarity/chemotaxis; EC 3.1.4.1; EC 3.6.1.9; Nucleotide Metabolism - purine; Phosphodiesterase; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Carbohydrate Metabolism - starch and sucrose; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cofactor and Vitamin Metabolism - riboflavin; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; basolateral plasma membrane; lysosomal membrane; integral to membrane; plasma membrane

Molecular Function: phosphodiesterase I activity; nucleotide diphosphatase activity; protein homodimerization activity; 3'-phosphoadenosine 5'-phosphosulfate binding; zinc ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; insulin receptor binding; polysaccharide binding; protein binding; nucleic acid binding; ATP binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; sequestering of triacylglycerol; generation of precursor metabolites and energy; vitamin metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; negative regulation of fat cell differentiation; bone remodeling; phosphate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; riboflavin metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; immune response; negative regulation of protein amino acid autophosphorylation; negative regulation of cell growth; regulation of bone mineralization; water-soluble vitamin metabolic process; inorganic diphosphate transport; negative regulation of glycogen biosynthetic process

Disease: Obesity; Cole Disease; Arterial Calcification, Generalized, Of Infancy, 1; Hypophosphatemic Rickets, Autosomal Recessive, 2; Diabetes Mellitus, Noninsulin-dependent