Mouse cathepsin S ELISA Kit | CTSS elisa kit

Mouse Cathepsin S, CTSS ELISA Kit

Cat. Num. AAA905650

• Reactivity: Mouse
• Synonyms: cathepsin S; Mouse Cathepsin S; CTSS ELISA Kit; Mouse Cathepsin S (CTSS) ELISA kit; MGC3886; ; CTSS elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse CTSS. No significant cross-reactivity or interference between mouse CTSS and analogues was observed.
• Sequence Length: 340

• Samples: Serum, plasma, tissue homogenates and cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: <7.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSS elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTSS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSS bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 510025483
• NCBI GeneID: 13040
• NCBI Accession #: NP_067256.4
• NCBI GenBank Nucleotide #: NM_021281.3
• UniProt Accession #: O70370; O54973; E9QK18
• Molecular Weight: 38,475 Da
• NCBI Official Full Name: cathepsin S isoform 2 preproprotein
• NCBI Official Synonym Full Names: cathepsin S
• NCBI Official Symbol: Ctss
• NCBI Protein Information: cathepsin S; Cat S
• UniProt Protein Name: Cathepsin S
• Protein Family: Cathepsin
• UniProt Gene Name: Ctss
• UniProt Synonym Gene Names: Cats
• UniProt Entry Name: CATS_MOUSE

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, which encode preproproteins that are proteolytically processed to generate mature protein products. This enzyme is secreted by antigen-presenting cells during inflammation and may induce pain and itch via activation of G-protein coupled receptors. Homozygous knockout mice for this gene exhibit impaired wound healing, reduced tumorigenesis in a pancreatic cancer model, and reduced pathogenesis in a myasthenia gravis model. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.27

Cellular Component: extracellular space; cell surface; membrane; intracellular membrane-bound organelle; lysosome

Molecular Function: peptidase activity; collagen binding; proteoglycan binding; hydrolase activity; fibronectin binding; cysteine-type endopeptidase activity; laminin binding; cysteine-type peptidase activity

Biological Process: collagen catabolic process; proteolysis involved in cellular protein catabolic process; regulation of sensory perception of pain; proteolysis; bone resorption; positive regulation of inflammatory response