Ctss recombinant protein

Ctss, 21-340aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206329

• Gene Names: Ctss; Cats
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Ctss; 21-340aa; Mouse; His tag; Baculovirus; Cathepsin S isoform 2; Cathepsin S; Ctss recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: VAMEQLQRDP TLDYHWDLWK KTHEKEYKDK NEEEVRRLIW EKNLKFIMIH NLEYSMGMHT YQVGMNDMGD MTNEEISCRM GALRISRQSP KTVTFRSYSN RTLPDTVDWR EKGCVTEVKY QGSCGACWAF SAVGALEGQL KLKTGKLISL SAQNLVDCSN EEKYGNKGCG GGYMTEAFQY IIDNGGIEAD ASYPYKAMDE KCHYNSKNRA ATCSRYIQLP FGDEDALKEA VATKGPVSVG IDASHSSFFF YKSGVYDDPS CTGNVNHGVL VVGYGTLDGK DYWLVKNSWG LNFGDQGYIR MARNNKNHCG IASYCSYPEI LEHHHHHHDY WLVKNSWGLN FGDQGYIRMA RNNKNHCGIA SYCSYPEILE HHHHHH
• Sequence Length: 340

• Applicable Applications for Ctss recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Ctss recombinant protein

Ctss, as known as cathepsin S isoform 2, is a lysosomal cysteine protease of the papain family. This protein plays a major role in the processing of the MHC class II-associated invariant chain. It has been implicated in the pathogenensis of several diseases such as Alzheimer s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system. Also, it is less abundant in tissues than Cathepsins B, L and H. The highest levels of this protein have been found in lymph nodes, spleen and phagocytic cells. Recombinant mouse Ctss, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Ctss recombinant protein

Immunology

References

Zhao P., et al. (2014) J. Biol. Chem. 289:27215-27234.; Lafarge JC., et al. (2014) Diabetologia. 57:1674-1683.

NCBI and Uniprot Product Information

• NCBI GI #: 510025483
• NCBI GeneID: 13040
• NCBI Accession #: NP_067256
• NCBI GenBank Nucleotide #: NM_021281.3
• UniProt Accession #: O70370; O54973; E9QK18
• Molecular Weight: 37.2kDa (328aa) 28-40KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: cathepsin S isoform 2 preproprotein
• NCBI Official Synonym Full Names: cathepsin S
• NCBI Official Symbol: Ctss
• NCBI Official Synonym Symbols: Cats
• NCBI Protein Information: cathepsin S
• UniProt Protein Name: Cathepsin S
• Protein Family: Cathepsin
• UniProt Gene Name: Ctss
• UniProt Synonym Gene Names: Cats

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, which encode preproproteins that are proteolytically processed to generate mature protein products. This enzyme is secreted by antigen-presenting cells during inflammation and may induce pain and itch via activation of G-protein coupled receptors. Homozygous knockout mice for this gene exhibit impaired wound healing, reduced tumorigenesis in a pancreatic cancer model, and reduced pathogenesis in a myasthenia gravis model. [provided by RefSeq, Aug 2015]

Uniprot Description

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.