Rabbit anti-Human SAR1B Polyclonal Antibody | anti-SAR1B antibody

SAR1B, ID (SAR1B, SARA2, SARB, GTP-binding protein SAR1b, GTP-binding protein B) (FITC)

Cat. Num. AAA6345033

• Gene Names: SAR1B; ANDD; CMRD; GTBPB; SARA2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SAR1B; Polyclonal Antibody; ID (SAR1B; SARA2; SARB; GTP-binding protein SAR1b; GTP-binding protein B) (FITC); GTP-binding protein B; anti-SAR1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SAR1B antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SAR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-124 amino acids from the Central region of human SAR1B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(SAR1B antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SAR1B antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(SAR1B Antibody (Center) western blot analysis in MCF-7,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SAR1B antibody detected the SAR1B protein (arrow).)

Flow Cytometry (FC/FACS)

(SAR1B Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SAR1B antibody

The protein encoded by this gene is a small GTPase that acts as a homodimer. The encoded protein is activated by the guanine nucleotide exchange factor PREB and is involved in protein transport from the endoplasmic reticulum to the Golgi. This protein is part of the COPII coat complex. Defects in this gene are a cause of chylomicron retention disease (CMRD), also known as Anderson disease (ANDD). Two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-SAR1B antibody

Antibodies; Binding Proteins

References

Cefalu, A.B., et al. Metab. Clin. Exp. 59(4):463-467(2010). Silvain, M., et al. Clin. Genet. 74(6):546-552(2008). Charcosset, M., et al. Mol. Genet. Metab. 93(1):74-84(2008). Jardim, D.L., et al. J. Biochem. Mol. Biol. 38(3):328-333(2005). Jones, B., et al. Nat. Genet. 34(1):29-31(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 7705827
• NCBI GeneID: 51128
• NCBI Accession #: NP_057187.1; NP_001028675.1
• NCBI GenBank Nucleotide #: NM_016103.3
• UniProt Accession #: Q9Y6B6
• NCBI Official Full Name: GTP-binding protein SAR1b
• NCBI Official Synonym Full Names: secretion associated Ras related GTPase 1B
• NCBI Official Symbol: SAR1B
• NCBI Official Synonym Symbols: ANDD; CMRD; GTBPB; SARA2
• NCBI Protein Information: GTP-binding protein SAR1b
• UniProt Protein Name: GTP-binding protein SAR1b
• Protein Family: GTP-binding protein
• UniProt Gene Name: SAR1B
• UniProt Synonym Gene Names: SARA2; SARB; GTBPB
• UniProt Entry Name: SAR1B_HUMAN

NCBI Description

The protein encoded by this gene is a small GTPase that acts as a homodimer. The encoded protein is activated by the guanine nucleotide exchange factor PREB and is involved in protein transport from the endoplasmic reticulum to the Golgi. This protein is part of the COPII coat complex. Defects in this gene are a cause of chylomicron retention disease (CMRD), also known as Anderson disease (ANDD). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

SAR1B: Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD); also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets. Belongs to the small GTPase superfamily. SAR1 family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, SAR1; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: endoplasmic reticulum membrane; cytosol

Molecular Function: GTPase activity; GTP binding; metal ion binding

Biological Process: COPII coating of Golgi vesicle; intracellular protein transport; ER to Golgi vesicle-mediated transport; antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Disease: Chylomicron Retention Disease