Rabbit anti-Mouse Cathepsin L Polyclonal Antibody | anti-CTSL antibody

Anti-Cathepsin L Antibody, Rabbit Polyclonal

Cat. Num. AAA8110776

• Gene Names: Ctsl; fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
• Reactivity: Mouse
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Cathepsin L; Polyclonal Antibody; Anti-Cathepsin L Antibody; Rabbit Polyclonal; Cathepsin L/CTSL Antibody; Rabbit PAb; Antigen Affinity Purified; cathepsin L; Anti-1190035F06Rik Antibody; Anti-Ctsl1 Antibody; Anti-fs Antibody; Anti-MEP Antibody; Anti-nkt Antibody; anti-CTSL antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Cathepsin L/CTSL
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CTSL antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000
• Immunogen: Recombinant Mouse Cathepsin L/CTSL Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Cathepsin L/CTSL (rM Cathepsin L/CTSL). Cathepsin L/CTSL specific IgG was purified by Mouse Cathepsin L/CTSL affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CTSL in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CTSL in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-CTSL antibody

Cathepsin L is a lysosomal cysteine protease that plays a major role in intracellular protein catabolism, and is potent in degrading collagen, laminin, elastin, as well as alpha-1 protease inhibitor and other structural proteins of basement membranes. It is secreted by liver flukes at all stages of their development in the mammalian host, are believed to play important roles in facilitating parasite migration (tissue degradation), feeding and immuno-evasion. Like many proteases, Cathepsin L is synthesized as an inactive preproenzyme, and cleavage of the 96-residue proregion is necessary to generate the fully active 221-residue mature enzyme. Studies have demonstrated that cleavage of the proregion occur autocatalytically under acidic conditions. The enzyme takes part in nutrient acquisition by catabolizing host proteins to absorbable peptides, facilitates the migration of the parasite through the host intestine and liver by cleaving interstitial matrix proteins such as fibronectin, laminin and native collagen and is implicated in the inactivation of host immune defenses by cleaving immunoglobulins. Recently, Cathepsin L has been shown to suppress Th1 immune response in infected laboratory animals making them susceptible to concurrent bacterial infections. Cathepsin L is synthesized in large amounts and secreted by many malignantly transformed cells, and induced by growth factors and tumor promoters. In addition to its role in protein degradation, evidence has accumulated for the participation of Cathepsin L in various physiological and pathological processes, such as tumor invasion and metastasis, bone resorption, spermatogenesis, and arthritis. Accordingly, Cathepsin L may prove useful as a or prognostic marker of human tumor malignancy.

References

Mulcahy G, et al. (2001) Cathepsin L proteinases as vaccines against infection with Fasciola hepatica (liver fluke) in ruminants. Res Vet Sci. 70(1): 83-6.Dixit AK, et al. (2008) Immunodiagnostic/protective role of cathepsin L cysteine proteinases secreted by Fasciola species. Vet Parasitol. 154(3-4): 177-84.Leto G, et al. (2010) Cathepsin L in metastatic bone disease: therapeutic implications. Biol Chem. 391(6): 655-64.

NCBI and Uniprot Product Information

• NCBI GI #: 6753558
• NCBI GeneID: 13039
• NCBI Accession #: NP_034114.1
• NCBI GenBank Nucleotide #: NM_009984.4
• UniProt Accession #: P06797; Q91UZ0
• Molecular Weight: 37,547 Da
• NCBI Official Full Name: cathepsin L1 preproprotein
• NCBI Official Synonym Full Names: cathepsin L
• NCBI Official Symbol: Ctsl
• NCBI Official Synonym Symbols: fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
• NCBI Protein Information: cathepsin L1
• UniProt Protein Name: Cathepsin L1
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsl
• UniProt Synonym Gene Names: Ctsl1; MEP

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSL2: Cysteine protease. May have an important role in corneal physiology. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.15; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13 B3|13 33.26 cM

Cellular Component: apical part of cell; cytoplasm; cytoplasmic vesicle; external side of plasma membrane; extracellular space; lysosome; microvillus; neuron projection; nucleolus; nucleus; perikaryon; secretory granule; vacuole

Molecular Function: aminopeptidase activity; cysteine-type carboxypeptidase activity; cysteine-type endopeptidase activity; histone binding; kininogen binding; peptide binding; protein binding; protein complex binding

Biological Process: catagen; cell communication; decidualization; hair follicle morphogenesis; protein autoprocessing; protein processing; proteolysis; proteolysis involved in cellular protein catabolic process