Cathepsin L / CTSL Recombinant Protein | CTSL recombinant protein

Recombinant Mouse Cathepsin L / CTSL Protein (His tag)

Cat. Num. AAA2547212

• Gene Names: Ctsl; fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: Cathepsin L / CTSL; Recombinant Mouse Cathepsin L / CTSL Protein (His tag); 1190035F06Rik; Ctsl1; MEP; fs; nkt; CTSL recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5
• Sequence Length: 334

• Application Notes: The secreted recombinant mouse CTSL (pro form) consists of 328 amino acids and has a calculated molecular mass of 37.3 kDa. The recombinant protein migrates as several bands probably including the pro form, mature form, the pro peptide and the cleaved heavy chain in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Thr 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for CTSL recombinant protein

Background: Cathepsin L is a lysosomal cysteine protease that plays a major role in intracellular protein catabolism, and is potent in degrading collagen, laminin, elastin, as well as alpha-1 protease inhibitor and other structural proteins of basement membranes. It is secreted by liver flukes at all stages of their development in the mammalian host, are believed to play important roles in facilitating parasite migration (tissue degradation), feeding and immuno-evasion. Like many proteases, Cathepsin L is synthesized as an inactive preproenzyme, and cleavage of the 96-residue proregion is necessary to generate the fully active 221-residue mature enzyme. Studies have demonstrated that cleavage of the proregion occur autocatalytically under acidic conditions. The enzyme takes part in nutrient acquisition by catabolizing host proteins to absorbable peptides, facilitates the migration of the parasite through the host intestine and liver by cleaving interstitial matrix proteins such as fibronectin, laminin and native collagen and is implicated in the inactivation of host immune defenses by cleaving immunoglobulins. Recently, Cathepsin L has been shown to suppress Th1 immune response in infected laboratory animals making them susceptible to concurrent bacterial infections. Cathepsin L is synthesized in large amounts and secreted by many malignantly transformed cells, and induced by growth factors and tumor promoters. In addition to its role in protein degradation, evidence has accumulated for the participation of Cathepsin L in various physiological and pathological processes, such as tumor invasion and metastasis, bone resorption, spermatogenesis, and arthritis. Accordingly, Cathepsin L may prove useful as a prognostic marker of human tumor malignancy.

Description: A DNA sequence encoding the mouse CTSL (P06797) (Met 1-Asn 334) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 6753558
• NCBI GeneID: 13039
• NCBI Accession #: NP_034114.1
• NCBI GenBank Nucleotide #: NM_009984.4
• UniProt Accession #: P06797; Q91UZ0
• Molecular Weight: 37,547 Da
• NCBI Official Full Name: cathepsin L1 preproprotein
• NCBI Official Synonym Full Names: cathepsin L
• NCBI Official Symbol: Ctsl
• NCBI Official Synonym Symbols: fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
• NCBI Protein Information: cathepsin L1
• UniProt Protein Name: Cathepsin L1
• UniProt Gene Name: Ctsl
• UniProt Synonym Gene Names: Ctsl1; MEP

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSL2: Cysteine protease. May have an important role in corneal physiology. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.15; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13 B3|13 33.26 cM

Cellular Component: apical part of cell; cytoplasm; cytoplasmic vesicle; external side of plasma membrane; extracellular space; lysosome; microvillus; neuron projection; nucleolus; nucleus; perikaryon; secretory granule; vacuole

Molecular Function: aminopeptidase activity; cysteine-type carboxypeptidase activity; cysteine-type endopeptidase activity; histone binding; kininogen binding; peptide binding; protein binding; protein complex binding

Biological Process: catagen; cell communication; decidualization; hair follicle morphogenesis; protein autoprocessing; protein processing; proteolysis; proteolysis involved in cellular protein catabolic process