CTSL recombinant protein

Recombinant Mouse CTSL Protein

Gene Names

Ctsl; fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik

Reactivity

Mouse

Applications

ELISA, Western Blot, SDS-Page

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Synonyms

CTSL; Recombinant Mouse CTSL Protein; Ctsl1; Cathepsin L1; Cathepsin L; Major excreted protein; MEP; p39 cysteine proteinase; CTSL recombinant protein

Ordering
Cat. Num. AAA8248887 For Research Use Only!

Host

Human cells

Reactivity

Mouse

Purity/Purification

Greater than 95% as determined by reducing SDS-PAGE.

Form/Format

Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Sequence

TPKFDQTFSA EWHQWKSTHR RLYGTNEEEW RRAIWEKNMR MIQLHNGEYS NGQHGFSMEM NAFGDMTNEE FRQVVNGYRH QKHKKGRLFQ EPLMLKIPKS VDWREKGCVT PVKNQGQCGS CWAFSASGCL EGQMFLKTGK LISLSEQNLV DCSHAQGNQG CNGGLMDFAF QYIKENGGLD SEESYPYEAK DGSCKYRAEF AVANDTGFVD IPQQEKALMK AVATVGPISV AMDASHPSLQ FYSSGIYYEP NCSSKNLDHG VLLVGYGYEG TDSNKNKYWL VKNSWGSEWG MEGYIKIAKD RDNHCGLATA ASYPVVNVDH HHHHH

Sequence Length

334

Applicable Applications for CTSL recombinant protein

ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)

Endotoxin

Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.

Directions for Use

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Preparation and Storage

Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CTSL recombinant protein

Recombinant Mouse CTSL Protein is produced by our mammalian expression system and the target gene encoding Thr18-Asn334 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

NCBI GI #

6753558

NCBI GeneID

13039

NCBI Accession #

NP_034114.1

NCBI GenBank Nucleotide #

NM_009984.4

UniProt Accession #

P06797; Q91UZ0

Molecular Weight

37,547 Da

NCBI Official Full Name

cathepsin L1 preproprotein

NCBI Official Synonym Full Names

cathepsin L

NCBI Official Symbol

Ctsl

NCBI Official Synonym Symbols

fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik

NCBI Protein Information

cathepsin L1

UniProt Protein Name

Cathepsin L1

UniProt Gene Name

Ctsl

UniProt Synonym Gene Names

Ctsl1; MEP

UniProt Entry Name

CATL1_MOUSE

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSL2: Cysteine protease. May have an important role in corneal physiology. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.15; Protease; Secreted; Secreted, signal peptide

Cellular Component: apical part of cell; cytoplasm; cytoplasmic vesicle; external side of plasma membrane; extracellular space; lysosome; microvillus; neuron projection; nucleolus; nucleus; perikaryon; secretory granule; vacuole

Molecular Function: aminopeptidase activity; cysteine-type carboxypeptidase activity; cysteine-type endopeptidase activity; histone binding; kininogen binding; peptide binding; protein binding; protein complex binding

Biological Process: catagen; cell communication; decidualization; hair follicle morphogenesis; protein autoprocessing; protein processing; proteolysis; proteolysis involved in cellular protein catabolic process

Research Articles on CTSL

1. findings suggest that single chain-cathepsin L is biologically active in promoting Th17 generation and is counter-regulated by serpinB1 and secondarily by asparagine endopeptidase.

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Product Notes

The CTSL ctsl (Catalog #AAA8248887) is a Recombinant Protein produced from Human cells and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Mouse CTSL Protein reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CTSL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS). Researchers should empirically determine the suitability of the CTSL ctsl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TPKFDQTFSA EWHQWKSTHR RLYGTNEEEW RRAIWEKNMR MIQLHNGEYS NGQHGFSMEM NAFGDMTNEE FRQVVNGYRH QKHKKGRLFQ EPLMLKIPKS VDWREKGCVT PVKNQGQCGS CWAFSASGCL EGQMFLKTGK LISLSEQNLV DCSHAQGNQG CNGGLMDFAF QYIKENGGLD SEESYPYEAK DGSCKYRAEF AVANDTGFVD IPQQEKALMK AVATVGPISV AMDASHPSLQ FYSSGIYYEP NCSSKNLDHG VLLVGYGYEG TDSNKNKYWL VKNSWGSEWG MEGYIKIAKD RDNHCGLATA ASYPVVNVDH HHHHH. It is sometimes possible for the material contained within the vial of "CTSL, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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