TrkC Active Protein | NTRK3 active protein

TrkC Protein, Human, Recombinant (His & hFc Tag)

Cat. Num. AAA8119964

• Gene Names: NTRK3; TRKC; gp145(trkC)
• Purity: >98% as determined by SDS-PAGE
• Synonyms: TrkC; TrkC Protein; Human; Recombinant (His & hFc Tag); Human TrkC/NTRK3 Protein (His & Fc Tag); gp145(trkC) Protein; TRKC Protein; neurotrophic tyrosine kinase; receptor; type 3; NTRK3 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp428

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Cys 32
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met1-Asp428) of human TrkC (NP_001007157.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Immobilized human TrKc-Fch at 10ug/ml (100uL/well) can bind biotinylated human NT3. The EC50 of biotinylated human NT3 is 23.4-54.6ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Immobilized human TrKc-Fch at 10 ug/ml (100 ul/well) can bind biotinylated human NT3, The EC50 of biotinylated human NT3 is 23.4-54.6 ng/ml.)

Related Product Information for NTRK3 active protein

Background: NT-3 growth factor receptor is also known as neurotrophic tyrosine kinase receptor type 3 or TrkC tyrosine kinase or Trk-C receptor, is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC/NTRK3 is widely expressed in the developing and adult nervous system. In later embryonic development, TrkC/NTRK3 is expressed in various structures of the CNS including the caudate-putamen, septal nuclei, cerebellum, and brainstem. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. In the PNS, The trkC hybridization appears to correlate, both temporally and spatially, with the outgrowth of axons toward their peripheral targets. TrkC/NTRK3 is widely expressed in the three identified branches of the mammalian nervous system and appears to correlate with the expression of NT-3, its cognate ligand. The apparent colocalization of trkC transcripts with NT-3 raises the possibility this neurotrophin exerts its trophic effects by a paracrine and/or autocrine mechanism. Signaling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in the TrkC encoding gene have been associated with medulloblastomas, secretory breast carcinomas, and other cancers.

Product Categories/Family for NTRK3 active protein

Receptor Tyrosine Kinases (RTKs) Cancer Drug Targets

References

Tessarollo L, et al. (1993) trkC, a receptor for neurotrophin-3, is widely expressed in the developing nervous system and in non-neuronal tissues. Development. 118(2): 463-75.
Lamballe F, et al. (1994) Developmental expression of trkC, the neurotrophin-3 receptor, in the mammalian nervous system. J Neurosci. 14(1): 14-28.
Klein R, et al. (1994) Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements. Nature. 368(6468): 249-51.

NCBI and Uniprot Product Information

• NCBI GI #: 55956794
• NCBI GeneID: 4916
• NCBI Accession #: NP_001007157.1
• NCBI GenBank Nucleotide #: NM_001007156.2
• UniProt Accession #: Q16288; O75682; Q12827; Q16289
• Molecular Weight: 839
• NCBI Official Full Name: NT-3 growth factor receptor isoform c
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 3
• NCBI Official Symbol: NTRK3
• NCBI Official Synonym Symbols: TRKC; gp145(trkC)
• NCBI Protein Information: NT-3 growth factor receptor; GP145-TrkC; ETV6-NTRK3 fusion; tyrosine kinase receptor C; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein
• UniProt Protein Name: NT-3 growth factor receptor
• Protein Family: NT-3 growth factor receptor
• UniProt Gene Name: NTRK3
• UniProt Synonym Gene Names: TRKC; Trk-C
• UniProt Entry Name: NTRK3_HUMAN

Uniprot Description

TrkC: a receptor tyrosine kinase of the TRK family. Receptor for neurotrophin-3 (NT-3). Known substrates for the trk receptors are SHC, PI-3 kinase, and PLCG1. Four alternatively spliced isoforms are known.

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; Trk family

Chromosomal Location of Human Ortholog: 15q25

Cellular Component: integral to plasma membrane; cytoplasm; receptor complex

Molecular Function: neurotrophin binding; protein binding; ephrin receptor binding; p53 binding; transmembrane receptor protein tyrosine kinase activity; neurotrophin receptor activity; ATP binding

Biological Process: circadian rhythm; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; positive regulation of positive chemotaxis; activation of MAPK activity; positive regulation of axon extension involved in regeneration; protein amino acid autophosphorylation; activation of protein kinase B; neuron migration; positive regulation of peptidyl-serine phosphorylation; response to ethanol; negative regulation of protein amino acid phosphorylation; positive regulation of cell proliferation; negative regulation of astrocyte differentiation; modification by virus of host cell transcription; neuron fate specification; response to axon injury; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration; response to corticosterone stimulus