Rabbit anti-Mouse Mab21 Domain Containing Protein 1 Polyclonal Antibody | anti-MB21D1 antibody

Polyclonal Antibody to Mab21 Domain Containing Protein 1 (MB21D1)

Cat. Num. AAA2027341

• Gene Names: Mb21d1; cGAS; E330016A19Rik
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Mab21 Domain Containing Protein 1; Polyclonal Antibody; Polyclonal Antibody to Mab21 Domain Containing Protein 1 (MB21D1); anti-MB21D1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against MB21D1. It has beenselected for its ability to recognize MB21D1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 522

• Applicable Applications for anti-MB21D1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.5-5ug/mL; Immunocytochemistry in formalin fixed cells: 5-20ug/mL; Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL; Immunohistochemistry in paraffin section: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Fragment: MB21D1 (Glu211~Lys395)
• Organism Species: Mus musculus (Mouse)
• Cross Reactivity: Human, Mouse
• Conjugate: No Conjugate
• Immunogen: Recombinant MB21D1 (Glu211~Lys395) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2085636
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human THP-1 Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant MB21D1, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Oviduct Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)

NCBI and Uniprot Product Information

• NCBI GI #: 116812912
• NCBI GeneID: 214763
• NCBI Accession #: NP_775562.2
• NCBI GenBank Nucleotide #: NM_173386.5
• UniProt Accession #: Q8C6L5; Q3ULW3
• Molecular Weight: 58,194 Da
• NCBI Official Full Name: cyclic GMP-AMP synthase
• NCBI Official Synonym Full Names: Mab-21 domain containing 1
• NCBI Official Symbol: Mb21d1
• NCBI Official Synonym Symbols: cGAS; E330016A19Rik
• NCBI Protein Information: cyclic GMP-AMP synthase
• UniProt Protein Name: Cyclic GMP-AMP synthase
• Protein Family: Cyclic GMP-AMP synthase
• UniProt Gene Name: Mb21d1
• UniProt Synonym Gene Names: m-cGAS

NCBI Description

The protein encoded by this gene is a DNA binding cytosolic protein that catalyzes the synthesis of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) after sensing the presence of DNA in the cytoplasm. cGAMP binds another protein, Stimulator of interferon genes (STING), leading to the induction of interferons, and a host immune response. Reduced expression of this gene inhibits interferon induction in the presence of some viral infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

cGAS: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.

Protein type: EC 2.7.7.86

Chromosomal Location of Human Ortholog: 9|9 E1

Cellular Component: cytoplasm; cytosol

Molecular Function: ATP binding; chromatin binding; DNA binding; double-stranded DNA binding; GTP binding; metal ion binding; nucleotide binding; nucleotidyltransferase activity; protein binding; transferase activity

Biological Process: activation of innate immune response; cellular response to DNA damage stimulus; cellular response to exogenous dsRNA; cyclic nucleotide biosynthetic process; defense response to virus; immune system process; innate immune response; paracrine signaling; positive regulation of cellular senescence; positive regulation of defense response to virus by host; positive regulation of interferon type I production