Cystatin F/CST7 Active Protein | CST7 active protein

Cystatin F/CST7 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120042

• Gene Names: CST7; CMAP
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Cystatin F/CST7; Cystatin F/CST7 Protein; Human; Recombinant (hFc Tag); Human Cystatin 7/CST7 Protein (Fc Tag); CMAP Protein; cystatin F (leukocystatin); CST7 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His145

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 20
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human Cystatin F precursor (NP_003641.3)(Met 1-His 145) was expressed with the fused Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is <5nM.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CST7 active protein

Background: The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new identification and prevention approach of certain human carcinomas metastasis.

References

Halfon S., et al.,(1998), Leukocystatin, a new class II cystatin expressed selectively by hematopoietic cells. J. Biol. Chem. 273:16400-16408.Ni J., et al., (1998), Cystatin F is a glycosylated human low molecular weight cysteine proteinase inhibitor.J. Biol. Chem. 273:24797-24804.Morita M., et al.,(2000), Genomic construct and mapping of the gene for CMAP (leukocystatin/cystatin F, CST7) and identification of a proximal novel gene, BSCv (C20orf3).Genomics 67:87-91.

NCBI and Uniprot Product Information

• NCBI GI #: 262263314
• NCBI GeneID: 8530
• NCBI Accession #: NP_003641.3
• NCBI GenBank Nucleotide #: NM_003650.3
• UniProt Accession #: O76096; Q6FH95; Q7Z4J8; Q9UED4
• Molecular Weight: 16,454 Da
• NCBI Official Full Name: cystatin-F
• NCBI Official Synonym Full Names: cystatin F
• NCBI Official Symbol: CST7
• NCBI Official Synonym Symbols: CMAP
• NCBI Protein Information: cystatin-F
• UniProt Protein Name: Cystatin-F
• Protein Family: Cystatin
• UniProt Gene Name: CST7
• UniProt Synonym Gene Names: CMAP
• UniProt Entry Name: CYTF_HUMAN

Uniprot Description

CST7: Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system. Belongs to the cystatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space

Molecular Function: endopeptidase inhibitor activity; protease binding; protein binding

Biological Process: immune response