Cystatin 7 / CST7 Recombinant Protein | CST7 recombinant protein

Recombinant Human Cystatin 7 / CST7 Protein (Fc tag)

Cat. Num. AAA2546465

• Gene Names: CST7; CMAP
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Cystatin 7 / CST7; Recombinant Human Cystatin 7 / CST7 Protein (Fc tag); CMAP; CST7; CST7 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
• Sequence Length: 145

• Application Notes: The recombinant human Cystatin-F/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 364 amino acids and predicts a molecular mass of 41.3 kDa. As a result of glycosylation, the rh Cystatin-F/Fc monomer migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CST7 recombinant protein

Background: The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain type s of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new identification and prevention approach of certain human carcinomas metastasis.

Description: A DNA sequence encoding the human Cystatin F precursor (NP_003641.3) (Met 1-His 145) was expressed with the fused Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 262263314
• NCBI GeneID: 8530
• NCBI Accession #: NP_003641.3
• NCBI GenBank Nucleotide #: NM_003650.3
• UniProt Accession #: O76096; Q6FH95; Q7Z4J8; Q9UED4
• Molecular Weight: 16,454 Da
• NCBI Official Full Name: cystatin-F
• NCBI Official Synonym Full Names: cystatin F
• NCBI Official Symbol: CST7
• NCBI Official Synonym Symbols: CMAP
• NCBI Protein Information: cystatin-F
• UniProt Protein Name: Cystatin-F
• Protein Family: Cystatin
• UniProt Gene Name: CST7
• UniProt Synonym Gene Names: CMAP

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been observed in various human cancer cell lines established from malignant tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

CST7: Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system. Belongs to the cystatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space

Molecular Function: endopeptidase inhibitor activity; protease binding; protein binding

Biological Process: immune response