Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) Recombinant Protein | MCSP recombinant protein

Recombinant Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP)

Cat. Num. AAA2009340

• Gene Names: Cspg4; Ng2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP); Recombinant Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP); MCSP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-GV FQLSMSDGAS PPIPMSLAVD VLPSTIEVQL RAPLEVPQAL GRSSLSRQQL QVISDREEPD VAYRLTQGPL YGQVLVGGQP ASAFSQLQVD QGDVVFAFTN FSSSQDHFKV LALARGVNAS ATVNVTVQAL LHVWAGGPWP QGTTLRLDPT VLDASELANR TGSMPRFRLL EGPRYGRVVR VSQGRAESRT NQLVEDFTQQ DLEEGRLGLE VGRPEGRSTG PTGDRLTLEL QATGVPPAVA LLDFATEPYH AAKFYKVTLL SVPEAARTET EKTGKSTPTG QPGQAASSPM PTVAKSGFLG FLEANMFSVI IPVCLVLLLL ALILPLLFYL RKRNKTGK
• Sequence Length: 2326

• Applicable Applications for MCSP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gly1919~Lys2258 (Accession # Q00657) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 13591932
• NCBI GeneID: 81651
• NCBI Accession #: NP_112284.1
• NCBI GenBank Nucleotide #: NM_031022.1
• UniProt Accession #: Q00657
• Molecular Weight: 63.6kDa
• NCBI Official Full Name: chondroitin sulfate proteoglycan 4
• NCBI Official Synonym Full Names: chondroitin sulfate proteoglycan 4
• NCBI Official Symbol: Cspg4
• NCBI Official Synonym Symbols: Ng2
• NCBI Protein Information: chondroitin sulfate proteoglycan 4; HSN tumor-specific antigen; membrane-spanning proteoglycan NG2; chondroitin sulfate proteoglycan NG2
• UniProt Protein Name: Chondroitin sulfate proteoglycan 4
• UniProt Gene Name: Cspg4
• UniProt Synonym Gene Names: Ng2
• UniProt Entry Name: CSPG4_RAT

NCBI Description

extracellular matrix protein secreted by many cell types; member of the large chondroitin sulfate proteoglycan (CSPG) family; may be involved with angiogenesis -dependent processes such as wound healing or tumor growth [RGD, Feb 2006]

Uniprot Description

NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: cell projection; cell surface; focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: collagen binding; signal transducer activity; protein kinase binding

Biological Process: cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; activation of MAPK activity; response to lithium ion; tissue remodeling; angiogenesis; signal transduction; glial cell migration; inflammatory response; transmembrane receptor protein tyrosine kinase signaling pathway; neuron remodeling