IDE recombinant protein

Recombinant IDE protein

Cat. Num. AAA389348

• Gene Names: IDE; INSULYSIN
• Synonyms: IDE; Recombinant IDE protein; Insulysin; or insulin-degrading enzyme; IDE recombinant protein

• Host: E Coli
• Form/Format: Recombinant IDE protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP

• Protein Species: Human
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant IDE protein gel 10% SDS-PAGE with Coomassie blue staining MW: 114.3 kDa Purity: >90%)

Related Product Information for IDE recombinant protein

Short Description: Full-length human IDE protein was expressed in E Coli (accession number NP_004960.2) with a C-terminal 6xHis-tag. The molecular weight of the protein is 114.3 kDa.

Background: IDE (Insulin Degrading Enzyme) is a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signaling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causative for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.

Product Categories/Family for IDE recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 155969707
• NCBI GeneID: 3416
• NCBI Accession #: NP_004960.2
• NCBI GenBank Nucleotide #: NM_004969.3
• UniProt Accession #: P14735; Q5T5N2; B2R721; B7ZAU2; D3DR35
• Molecular Weight: 54,240 Da
• NCBI Official Full Name: insulin-degrading enzyme isoform 1
• NCBI Official Synonym Full Names: insulin-degrading enzyme
• NCBI Official Symbol: IDE
• NCBI Official Synonym Symbols: INSULYSIN
• NCBI Protein Information: insulin-degrading enzyme; insulinase; insulin protease; Abeta-degrading protease
• UniProt Protein Name: Insulin-degrading enzyme
• Protein Family: IgM protease
• UniProt Gene Name: IDE
• UniProt Synonym Gene Names: Insulinase
• UniProt Entry Name: IDE_HUMAN

NCBI Description

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]

Uniprot Description

IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Nuclear receptor co-regulator; Cell surface; EC 3.4.24.56

Chromosomal Location of Human Ortholog: 10q23-q25

Cellular Component: nucleoplasm; peroxisomal matrix; extracellular space; cell surface; mitochondrion; cytoplasm; plasma membrane; peroxisome; cytosol; nucleus

Molecular Function: ubiquitin binding; protein homodimerization activity; zinc ion binding; beta-amyloid binding; metalloendopeptidase activity; ATPase activity; peptide binding; beta-endorphin binding; insulin binding; protein binding; glycoprotein binding; ATP binding; receptor binding

Biological Process: negative regulation of proteolysis; positive regulation of protein oligomerization; proteolysis involved in cellular protein catabolic process; viral reproduction; protein heterooligomerization; beta-amyloid metabolic process; insulin receptor signaling pathway; hormone catabolic process; determination of adult life span; proteolysis; protein homooligomerization; protein homotetramerization