Mouse anti-Human, Rat Moesin Monoclonal Antibody | anti-MSN antibody

Moesin Mouse Monoclonal Antibody

Cat. Num. AAA438223

• Gene Names: MSN; HEL70
• Reactivity: Human, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Moesin; Monoclonal Antibody; Moesin Mouse Monoclonal Antibody; Membrane-organizing extension spike protein; Moesin/anaplastic lymphoma kinase fusion protein; MSN/ALK fusion; anti-MSN antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM562
• Specificity: Recognizes 78kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 577

• Applicable Applications for anti-MSN antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cell Surface
• Immunogen: Recombinant human Moesin protein
• Hu-Chromosome Location: Xq11.1
• Positive Control: HT-29, CH3LC, or HUVEC cells. Uterus, placenta, tonsil (both B and T lymphocytes), skeletal muscle, thyroid, or kidney.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Placenta stained with Moesin Monoclonal Antibody (SPM562))

References

Lankes W et. al., Biochem Journal, 1988; 251:831-842. Schwartz-Albiez R et. al., European Journal Cell Biology, 1995; 67:189-198.

NCBI and Uniprot Product Information

• NCBI GI #: 4505257
• NCBI GeneID: 4478
• NCBI Accession #: NP_002435.1
• NCBI GenBank Nucleotide #: NM_002444.2
• UniProt Accession #: P26038
• Molecular Weight: 78kDa
• NCBI Official Full Name: moesin
• NCBI Official Synonym Full Names: moesin
• NCBI Official Symbol: MSN
• NCBI Official Synonym Symbols: HEL70
• NCBI Protein Information: moesin
• UniProt Protein Name: Moesin
• Protein Family: Moesin
• UniProt Gene Name: MSN
• UniProt Entry Name: MOES_HUMAN

NCBI Description

Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008]

Uniprot Description

Probably involved in connections of major cytoskeletal structures to the plasma membrane. May inhibit herpes simplex virus 1 infection at an early stage.