Integrin Alpha M (ITGaM) Recombinant Protein | ITGaM recombinant protein

Recombinant Integrin Alpha M (ITGaM)

Cat. Num. AAA2010786

• Gene Names: ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Integrin Alpha M (ITGaM); Recombinant Integrin Alpha M (ITGaM); ITGaM recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-NTIASKPPRD HVFQVNNFEA LKTIQNQLRE KIFAIEGTQT GSSSSFEHEM SQEGFSAAIT SNGPLLSTVG SYDWAGGVFL YTSKEKSTFI NMTRVDSDMN DAYLGYAAAI ILRNRVQSLV LGAPRYQHIG LVAMFRQNTG MWESNANVKG T
• Sequence Length: 1152

• Applicable Applications for ITGaM recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asn301~Thr451 (Accession # P11215) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 88501734
• NCBI GeneID: 3684
• NCBI Accession #: NP_000623.2
• NCBI GenBank Nucleotide #: NM_000632.3
• UniProt Accession #: P11215; Q4VAK0; Q4VAK1; Q4VAK2
• Molecular Weight: 43.7kDa
• NCBI Official Full Name: integrin alpha-M isoform 2
• NCBI Official Synonym Full Names: integrin, alpha M (complement component 3 receptor 3 subunit)
• NCBI Official Symbol: ITGAM
• NCBI Official Synonym Symbols: CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• NCBI Protein Information: integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit
• UniProt Protein Name: Integrin alpha-M
• Protein Family: Integrin
• UniProt Gene Name: ITGAM
• UniProt Synonym Gene Names: CD11B; CR3A
• UniProt Entry Name: ITAM_HUMAN

NCBI Description

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; plasma membrane; integrin complex; nucleus; external side of plasma membrane

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; opsonin binding; protein binding; protein heterodimerization activity; metal ion binding; glycoprotein binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; cellular extravasation; activated T cell proliferation; toll-like receptor signaling pathway; innate immune response; blood coagulation; cell adhesion; toll-like receptor 4 signaling pathway; leukocyte migration; leukocyte migration during inflammatory response

Disease: Systemic Lupus Erythematosus, Susceptibility To, 6