Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) Polyclonal Antibody | anti-MCSP antibody
Polyclonal Antibody to Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP)
MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-GV FQLSMSDGAS PPIPMSLAVD VLPSTIEVQL RAPLEVPQAL GRSSLSRQQL QVISDREEPD VAYRLTQGPL YGQVLVGGQP ASAFSQLQVD QGDVVFAFTN FSSSQDHFKV LALARGVNAS ATVNVTVQAL LHVWAGGPWP QGTTLRLDPT VLDASELANR TGSMPRFRLL EGPRYGRVVR VSQGRAESRT NQLVEDFTQQ DLEEGRLGLE VGRPEGRSTG PTGDRLTLEL QATGVPPAVA LLDFATEPYH AAKFYKVTLL SVPEAARTET EKTGKSTPTG QPGQAASSPM PTVAKSGFLG FLEANMFSVI IPVCLVLLLL ALILPLLFYL RKRNKTGK
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue))
NCBI and Uniprot Product Information
NCBI Description
extracellular matrix protein secreted by many cell types; member of the large chondroitin sulfate proteoglycan (CSPG) family; may be involved with angiogenesis -dependent processes such as wound healing or tumor growth [RGD, Feb 2006]
Uniprot Description
NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 8q24
Cellular Component: apical plasma membrane; cell projection; cell surface; extracellular exosome; extracellular matrix; focal adhesion; integral component of membrane; integral component of plasma membrane; intracellular; plasma membrane
Molecular Function: collagen binding; protein kinase binding; signal transducer activity
Biological Process: activation of MAPK activity; angiogenesis; cell proliferation; glial cell migration; inflammatory response; negative regulation of neuron projection development; neuron remodeling; positive regulation of peptidyl-tyrosine phosphorylation; response to lithium ion; signal transduction; tissue remodeling; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on MCSP
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Product Notes
The MCSP cspg4 (Catalog #AAA2001761) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MCSP cspg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-GV FQLSMSDGAS PPIPMSLAVD VLPSTIEVQL RAPLEVPQAL GRSSLSRQQL QVISDREEPD VAYRLTQGPL YGQVLVGGQP ASAFSQLQVD QGDVVFAFTN FSSSQDHFKV LALARGVNAS ATVNVTVQAL LHVWAGGPWP QGTTLRLDPT VLDASELANR TGSMPRFRLL EGPRYGRVVR VSQGRAESRT NQLVEDFTQQ DLEEGRLGLE VGRPEGRSTG PTGDRLTLEL QATGVPPAVA LLDFATEPYH AAKFYKVTLL SVPEAARTET EKTGKSTPTG QPGQAASSPM PTVAKSGFLG FLEANMFSVI IPVCLVLLLL ALILPLLFYL RKRNKTGK. It is sometimes possible for the material contained within the vial of "Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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