Flow Cytometry (FC/FACS)
(Flow cytometry analysis (intracellular staining) of cytokeratin expression in HeLa cells and in peripheral blood cells using anti-cytokeratin antibody (C-11) Alexa Fluor 488.)
Mouse anti-Mammalian Cytokeratins Monoclonal Antibody
Anti-Cytokeratins Alexa Fluor488
Reactivity
Mammalian
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunocytochemistry
Purity
The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
Cytokeratins; Monoclonal Antibody; Anti-Cytokeratins Alexa Fluor488; cytokeratin; CYK; CK; KRT; anti-Cytokeratins antibody
Host
Mouse
Reactivity
Mammalian
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
C-11
Specificity
The antibody C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
Purity/Purification
The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Concentration
0.1mg/ml (varies by lot)
Applicable Applications for anti-Cytokeratins antibody
Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
FC/FACS: Recommended dilution: 1ug/ml. Intracellular staining.
Conjugation
Alexa Fluor488
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (intracellular staining) of cytokeratin expression in HeLa cells and in peripheral blood cells using anti-cytokeratin antibody (C-11) Alexa Fluor 488.)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (intracellular staining) of cytokeratin expression in HeLa cells and in peripheral blood cells using anti-cytokeratin antibody (C-11) Alexa Fluor 488.)
Related Product Information for anti-Cytokeratins antibody
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40kDa and 68kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
References
*Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol. 1998 Aug;101(2):115-26., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER: Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats. Kidney Int. 2005 Dec;68(6):2572-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/16316332dopt=,*Vojtesek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/2483834,*Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/2463228dopt=A,*Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305dopt=A
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Product Notes
The Cytokeratins (Catalog #AAA1801948) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytokeratins Alexa Fluor488 reacts with Mammalian and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratins can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB), Immunocytochemistry (ICC). FC/FACS: Recommended dilution: 1ug/ml. Intracellular staining. Researchers should empirically determine the suitability of the Cytokeratins for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratins, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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