Rabbit anti-Human ADI1 Polyclonal Antibody | anti-ADI1 antibody

ADI1, ID (ADI1, MTCBP1, 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, Acireductone dioxygenase (Fe(2+)-requiring), Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, Submergence-induced protein-like factor)

Cat. Num. AAA6004971

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ADI1; Polyclonal Antibody; ID (ADI1; MTCBP1; 1; 2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; Acireductone dioxygenase (Fe(2+)-requiring); Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1; Submergence-induced protein-like factor); Anti -ADI1; anti-ADI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ADI1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: ADI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-116 amino acids from the Central region of human ADI1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADI1 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the ADI1 antibody detected the ADI1 protein (arrow).)

Related Product Information for anti-ADI1 antibody

This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20.

Product Categories/Family for anti-ADI1 antibody

Antibodies; Abs to Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 6323651
• NCBI GeneID: 855021
• NCBI Accession #: NP_013722.1
• NCBI GenBank Nucleotide #: NM_001182505.1
• UniProt Accession #: Q03677; D6VZI3
• Molecular Weight: 20,879 Da
• NCBI Official Full Name: acireductone dioxygenase (Ni2+-requiring)
• NCBI Official Symbol: ADI1
• NCBI Protein Information: acireductone dioxygenase (Ni2+-requiring)
• UniProt Protein Name: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
• Protein Family: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
• UniProt Gene Name: ADI1
• UniProt Synonym Gene Names: ARD; Fe-ARD
• UniProt Entry Name: MTND_YEAST

Uniprot Description

Function: Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Ref.5 Ref.7

Catalytic activity: 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate. Ref.5

Cofactor: Binds 1 iron ion per monomer. Can also use other divalent metal cations

By similarity.

Pathway: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6. HAMAP-Rule MF_03154

Subcellular location: Cytoplasm. Nucleus Ref.4 Ref.5.

Induction: By heat shock. Ref.6

Sequence similarities: Belongs to the acireductone dioxygenase (ARD) family.