VTI1A recombinant protein

VTI1A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121742

• Gene Names: VTI1A; MVti1; VTI1RP2; Vti1-rp2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: VTI1A; VTI1A Protein; Human; Recombinant (His Tag); Human VTI1A Protein (His Tag); MMDS3 Protein; MVti1 Protein; Vti1-rp2 Protein; VTI1RP2 Protein; vesicle transport through interaction with t-SNAREs 1A; VTI1A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg187

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human VTI1A (Q96AJ9-2)(Met1-Arg187) was expressed with an N-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for VTI1A recombinant protein

Background: Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) proteins form a complex that drives membrane fusion in eukaryotes. VTI1A and VTI1B are the only members of their SNARE subclass and the yeast homolog Vti1p is essential for cell survival. VTI1A is involved in regulating insulin-stimulated trafficking of secretory vesicles enriched with both GLUT4 (glucose transporter) and Acrp30 in adipocytes; it also plays key roles in neuronal development and in selectively maintaining spontaneous neurotransmitter release.

NCBI and Uniprot Product Information

• NCBI GI #: 113374156
• NCBI GeneID: 143187
• NCBI Accession #: NP_660207.2
• NCBI GenBank Nucleotide #: NM_145206.2
• UniProt Accession #: Q96AJ9; Q5W0D7; A2A307; B4E137
• Molecular Weight: 25,218 Da
• NCBI Official Full Name: vesicle transport through interaction with t-SNAREs homolog 1A
• NCBI Official Synonym Full Names: vesicle transport through interaction with t-SNAREs 1A
• NCBI Official Symbol: VTI1A
• NCBI Official Synonym Symbols: MVti1; VTI1RP2; Vti1-rp2
• NCBI Protein Information: vesicle transport through interaction with t-SNAREs homolog 1A; SNARE Vti1a-beta protein; vesicle transport v-SNARE protein Vti1-like 2
• UniProt Protein Name: Vesicle transport through interaction with t-SNAREs homolog 1A
• Protein Family: Vesicle transport through interaction with t-SNAREs
• UniProt Gene Name: VTI1A
• UniProt Entry Name: VTI1A_HUMAN

Uniprot Description

VTI1A: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non- conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence. Belongs to the VTI1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: SNARE complex; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; synaptic vesicle; Golgi stack; clathrin-coated vesicle; cell soma; perinuclear region of cytoplasm; late endosome membrane; integral to membrane; endosome

Molecular Function: SNAP receptor activity; SNARE binding

Biological Process: intra-Golgi vesicle-mediated transport; voluntary musculoskeletal movement; ER to Golgi vesicle-mediated transport; Golgi to vacuole transport; protein targeting to vacuole; vesicle fusion with Golgi apparatus; retrograde transport, endosome to Golgi

Disease: Multiple Mitochondrial Dysfunctions Syndrome 3