Rat GM-CSF ELISA Kit | CSF2 elisa kit

Rat GM-CSF ELISA Kit

Cat. Num. AAA8244703

• Gene Names: Csf2; Gmcsf; Gm-csf
• Reactivity: Rat
• Applications: ELISA
• Synonyms: GM-CSF; Rat GM-CSF ELISA Kit; GMCSF; Granulocyte-macrophage colony-stimulating factor; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; CSF2 elisa kit

• Reactivity: Rat
• Specificity: The Rat GM-CSF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat GM-CSF proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 144

• Applicable Applications for CSF2 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 7 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for CSF2 elisa kit

Principle of the Assay||The Rat GM-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat GM-CSF in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

NCBI and Uniprot Product Information

• NCBI GI #: 215273996
• NCBI GeneID: 116630
• UniProt Accession #: P48750
• Molecular Weight: 16,562 Da
• NCBI Official Full Name: Granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: Csf2
• NCBI Official Synonym Symbols: Gmcsf; Gm-csf
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• Protein Family: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: Csf2
• UniProt Synonym Gene Names: Csfgm; GM-CSF; CSF
• UniProt Entry Name: CSF2_RAT

NCBI Description

plays a role in alveolar epithelial fluid transport [RGD, Feb 2006]

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space

Molecular Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity

Biological Process: embryonic placenta development; epithelial cell proliferation; epithelial fluid transport; histamine secretion; immune response; macrophage activation; myeloid dendritic cell differentiation; negative regulation of cytolysis; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of interleukin-23 production; positive regulation of macrophage differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of cell proliferation; regulation of circadian sleep/wake cycle, sleep; regulation of gene expression; response to silicon dioxide