Human GM-CSF ELISA Kit | CSF2 elisa kit

Human GM-CSF ELISA Kit

Cat. Num. AAA8244592

• Gene Names: CSF2; GMCSF
• Reactivity: Human
• Applications: ELISA
• Synonyms: GM-CSF; Human GM-CSF ELISA Kit; GMCSF; Granulocyte-macrophage colony-stimulating factor; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; CSF2 elisa kit

• Reactivity: Human
• Specificity: The Human GM-CSF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human GM-CSF proteins within the range of 31.3 pg/ml - 2000 pg/ml.
• Sequence Length: 144

• Applicable Applications for CSF2 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates, Urine.
• Sensitivity: 7 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for CSF2 elisa kit

Principle of the Assay||The Human GM-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GM-CSF in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates, Urine. This assay employs an antibody specific for Human GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Granulocyte macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine with multiple effects on hematopoietic cells (1-4). It mobilizes CD34+ progenitor cells into the periphery and stimulates their proliferation, survival and differentiation into neutrophils,monocytes/ macrophages, eosinophils, and myeloid dendritic cells (4-8). On these terminallydifferentiated myeloid cells, GM-CSF is also needed for inducing their effector functions (7-10). In addition, GM-CSF has been shown to stimulate the proliferation and differentiation of the erythroid and megakaryoctye progenitor cells (4). GM-CSF is produced by a number of different cell types, including keratinocytes, mature and immature NK cells, type II alveolar cells), endothelial cells, monocytes, bone-marrow mesenchymal stem cells, CD4+ and CD8+ T cells, megakaryocytes, B cells, eosinophils, chondrocytes and fibroblasts. Human GM-CSF cDNA encodes a 144 amino acid (aa) residue precursor protein with a 17 aa putative signal peptide and a 127 aa mature protien (11 - 13). Natural GM-CSF is a monomer that contains both N- and O-linked glycosylation (14). Mature human GM-CSF shares approximately 55%, 63% and 68% aa sequence homology with mouse, rat and canine. GM-CSF, respectively. Human GM-CSF is not biologically active on mouse cells (11), but was reported to have some activity on canine cells (15). GM-CSF exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins. The presence of the spliced variants in the heteromeric receptor complex can regulate the functions of the complex (16).

NCBI and Uniprot Product Information

• NCBI GI #: 117561
• NCBI GeneID: 1437
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: Granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• Protein Family: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity; protein binding

Biological Process: activation of MAPKK activity; axon guidance; embryonic placenta development; epidermal growth factor receptor signaling pathway; epithelial fluid transport; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; macrophage activation; MAPKKK cascade; myeloid dendritic cell differentiation; negative regulation of cytolysis; nerve growth factor receptor signaling pathway; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of interleukin-23 production; positive regulation of tyrosine phosphorylation of Stat5 protein; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway