Human GM-CSF ELISA Kit | CSF2 elisa kit

Human GM-CSF PicoKine ELISA Kit

Cat. Num. AAA176000

• Gene Names: CSF2; GMCSF
• Reactivity: Human
• Synonyms: GM-CSF; Human GM-CSF PicoKine ELISA Kit; Granulocyte-macrophage colony-stimulating factor; colony stimulating factor 2 (granulocyte-macrophage); Colony stimulating factor; Colony stimulating factor 2 (granulocyte macrophage); CSF; CSF 2; CSF2; GMCSF; Granulocyte macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostin; Sargramostim; CSF2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human GM-CSF
• Sequence Length: 144

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human GM-CSF PicoKine ELISA Kit standard curve )

Related Product Information for CSF2 elisa kit

Principle of the assay: human GM-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GM-CSF has been precoated onto 96-well plates. Standards(E.coli,A18-E144) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GM-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GM-CSF amount of sample captured in plate.
Background: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene.1 Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells andacts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia.2 A complementary DNA for the T lymphocyte-derived lymphokine,GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.3

NCBI and Uniprot Product Information

• NCBI GI #: 117561
• NCBI GeneID: 1437
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: Granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• Protein Family: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; macrophage activation; embryonic placenta development; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; myeloid dendritic cell differentiation; immune response; positive regulation of DNA replication