Mouse GM-CSF ELISA Kit | CSF2 elisa kit

Mouse GM-CSF PicoKine ELISA Kit

Cat. Num. AAA175921

• Gene Names: Csf2; Csfgm; GMCSF; Gm-CSf; MGI-IGM
• Reactivity: Mouse
• Synonyms: GM-CSF; Mouse GM-CSF PicoKine ELISA Kit; Granulocyte-macrophage colony-stimulating factor; colony stimulating factor 2 (granulocyte-macrophage); Colony stimulating factor; Colony stimulating factor 2 (granulocyte macrophage); CSF; CSF 2; CSF2; GMCSF; Granulocyte macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostin; Sargramostim; CSF2 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Csf2
• Sequence Length: 141

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CSF2 elisa kit

Principle of the Assay: The Picokine™ Mouse Csf2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Csf2 with a 96-well strip plate that is pre-coated with antibody specific for Csf2. The detection antibody is a biotinylated antibody specific for Csf2. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Csf2 with immunogen: Expression system for standard: E.coli; Immunogen sequence: A18-K141. The kit is analytically validated with ready to use reagents. To measure Mouse Csf2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Csf2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Csf2 in the sample.

Background/Introduction: Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GMCSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GMCSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 117562
• NCBI GeneID: 12981
• UniProt Accession #: P01587
• Molecular Weight: 16,091 Da
• NCBI Official Full Name: Granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: Csf2
• NCBI Official Synonym Symbols: Csfgm; GMCSF; Gm-CSf; MGI-IGM
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor; CSF; put. GM-CSF; granulocyte-macrophage colony stimulating factor 2
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• Protein Family: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: Csf2
• UniProt Synonym Gene Names: Csfgm; GM-CSF; CSF
• UniProt Entry Name: CSF2_MOUSE

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; macrophage activation; embryonic placenta development; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of gene expression; positive regulation of interleukin-23 production; positive regulation of cell proliferation; immune response; myeloid dendritic cell differentiation; positive regulation of DNA replication; regulation of cell proliferation