Mouse Corticoliberin ELISA Kit | Crh elisa kit

Mouse Corticoliberin ELISA Kit

Cat. Num. AAA766023

• Gene Names: CRH; CRF; CRH1
• Reactivity: Mouse
• Synonyms: Corticoliberin; Mouse Corticoliberin ELISA Kit; Crh (Corticotropin Releasing Hormone)/Corticoliberin/Corticotropin-releasing factor/CRF/Corticotropin-releasing hormone; Crh elisa kit

• Reactivity: Mouse
• Sequence Length: 196

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Crh elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse Crh. During the reaction,Mouse Crh in the sample or standard competes with a fixed amount of Mouse Crh on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Mouse Crh. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse Crh in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503041
• NCBI GeneID: 1392
• NCBI Accession #: NP_000747.1
• NCBI GenBank Nucleotide #: NM_000756.3
• Molecular Weight: 21,422 Da
• NCBI Official Full Name: corticoliberin preproprotein
• NCBI Official Synonym Full Names: corticotropin releasing hormone
• NCBI Official Symbol: CRH
• NCBI Official Synonym Symbols: CRF; CRH1
• NCBI Protein Information: corticoliberin
• UniProt Protein Name: Corticoliberin
• Protein Family: Corticoliberin
• UniProt Gene Name: CRH
• UniProt Synonym Gene Names: CRF
• UniProt Entry Name: CRF_HUMAN

NCBI Description

This gene encodes a member of the corticotropin-releasing factor family. The encoded preproprotein is proteolytically processed to generate the mature neuropeptide hormone. In response to stress, this hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus, binds to corticotropin releasing hormone receptors and stimulates the release of adrenocorticotropic hormone from the pituitary gland. Marked reduction in this protein has been observed in association with Alzheimer's disease. Autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes, and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Nov 2015]

Uniprot Description

CRH: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: cytoplasm; extracellular region; extracellular space; perikaryon

Molecular Function: corticotropin-releasing hormone receptor 1 binding; corticotropin-releasing hormone receptor 2 binding; hormone activity; neuropeptide hormone activity; protein binding; receptor binding

Biological Process: adrenal gland development; associative learning; diterpenoid metabolic process; female pregnancy; glucocorticoid biosynthetic process; hypothalamus development; induction of apoptosis by hormones; inflammatory response; ion homeostasis; learning and/or memory; lung development; negative regulation of blood pressure; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of epinephrine secretion; negative regulation of luteinizing hormone secretion; parturition; positive regulation of adrenocorticotropic hormone secretion; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; positive regulation of cortisol secretion; positive regulation of protein amino acid phosphorylation; regulation of serotonin secretion; response to corticosterone stimulus; response to drug; response to estrogen stimulus; response to ethanol; response to ether; response to pain; signal transduction; synaptic transmission; synaptic transmission, dopaminergic

Disease: Acth Deficiency, Isolated