CREB3L1 recombinant protein

CREB3L1 Protein, Human, Recombinant (aa 396-519, His Tag)

Cat. Num. AAA8121215

• Gene Names: CREB3L1; OASIS
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CREB3L1; CREB3L1 Protein; Human; Recombinant (aa 396-519; His Tag); Human CREB3L1/OASIS Protein (aa 396-519; OASIS Protein; PSEC0238 Protein; cAMP responsive element binding protein 3-like 1; CREB3L1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu396-Ser519

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 396
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CREB3L1 (Q96BA8-1)(Glu396-Ser519) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CREB3L1 recombinant protein

Background: CREB3L1, also known as OASIS, is a cellular transcription factor synthesized as a membrane-bound precursor. It is a putative endoplasmic reticulum (ER) stress sensor in astrocytes with a mechanism of activation. OASIS mRNA expression was detected in pancreatic beta-cell lines and rodent islets, and the expression level was up-regulated by ER stress-inducing compounds. CREB3L1 may have a role in pancreas development. CREB3L1 may also play an important role in limiting virus spread by inhibiting proliferation of virus-infected cells. In vitro, CREB3L1 binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE. It may play a role in gliosis.

Product Categories/Family for CREB3L1 recombinant protein

AKT Signaling Pathway

References

Denard B. et al., 2011, Cell Host Microbe. 10 (1): 65-74. Debelenko LV. et al., 2011, 50 (12): 1054-62. Vellanki. et al., 2010, Endocrinology. 151 (9): 4146.

NCBI and Uniprot Product Information

• NCBI GI #: 16418355
• NCBI GeneID: 90993
• NCBI Accession #: NP_443086.1
• NCBI GenBank Nucleotide #: NM_052854.3
• UniProt Accession #: Q96BA8; Q8N2D5; Q96CP0
• Molecular Weight: 47,783 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 3-like protein 1
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 3-like 1
• NCBI Official Symbol: CREB3L1
• NCBI Official Synonym Symbols: OASIS
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 3-like protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 3-like protein 1
• Protein Family: Cyclic AMP-responsive element-binding protein 3-like protein
• UniProt Gene Name: CREB3L1
• UniProt Synonym Gene Names: OASIS; cAMP-responsive element-binding protein 3-like protein 1; OASIS
• UniProt Entry Name: CR3L1_HUMAN

NCBI Description

The protein encoded by this gene is normally found in the membrane of the endoplasmic reticulum (ER). However, upon stress to the ER, the encoded protein is cleaved and the released cytoplasmic transcription factor domain translocates to the nucleus. There it activates the transcription of target genes by binding to box-B elements. [provided by RefSeq, Jun 2013]

Uniprot Description

CREB3L1: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE. Belongs to the bZIP family. ATF subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transcription factor

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; nucleus

Molecular Function: chromatin binding; protein binding; sequence-specific DNA binding

Biological Process: multicellular organismal development; osteoblast differentiation; transcription from RNA polymerase II promoter; unfolded protein response