Rabbit anti-Human KCNJ1 Polyclonal Antibody | anti-KCNJ1 antibody

KCNJ1, CT (KCNJ1, ROMK1, ATP-sensitive inward rectifier potassium channel 1, ATP-regulated potassium channel ROM-K, Inward rectifier K(+) channel Kir1.1, Potassium channel, inwardly rectifying subfamily J member 1) (Azide free) (HRP)

Cat. Num. AAA6312482

• Gene Names: KCNJ1; ROMK; ROMK1; KIR1.1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KCNJ1; Polyclonal Antibody; CT (KCNJ1; ROMK1; ATP-sensitive inward rectifier potassium channel 1; ATP-regulated potassium channel ROM-K; Inward rectifier K(+) channel Kir1.1; Potassium channel; inwardly rectifying subfamily J member 1) (Azide free) (HRP); inwardly rectifying subfamily J member 1; anti-KCNJ1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 372

• Applicable Applications for anti-KCNJ1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KCNJ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-355 amino acids from the C-terminal region of human KCNJ1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KCNJ1 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the KCNJ1 antibody detected the KCNJ1 protein (arrow).)

Related Product Information for anti-KCNJ1 antibody

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-KCNJ1 antibody

Antibodies; Ion Channel

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Lin, D.H., et al. J. Biol. Chem. 284(43):29614-29624(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 148539892
• NCBI GeneID: 3758
• NCBI Accession #: NP_722449.2
• NCBI GenBank Nucleotide #: NP_722449.2
• UniProt Accession #: P48048
• NCBI Official Full Name: ATP-sensitive inward rectifier potassium channel 1 isoform b
• NCBI Official Synonym Full Names: potassium inwardly rectifying channel subfamily J member 1
• NCBI Official Symbol: KCNJ1
• NCBI Official Synonym Symbols: ROMK; ROMK1; KIR1.1
• NCBI Protein Information: ATP-sensitive inward rectifier potassium channel 1
• UniProt Protein Name: ATP-sensitive inward rectifier potassium channel 1
• Protein Family: ATP-sensitive inward rectifier potassium channel
• UniProt Gene Name: KCNJ1
• UniProt Synonym Gene Names: ROMK1
• UniProt Entry Name: IRK1_HUMAN

NCBI Description

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.

Enzyme regulation: Inhibited by WNK3. Ref.10

Subunit structure: Interacts with SGK1 and SLC9A3R2/NHERF2. Ref.8

Subcellular location: Cell membrane; Multi-pass membrane protein. Note: Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane. Ref.9

Tissue specificity: In the kidney and pancreatic islets. Lower levels in skeletal muscle, pancreas, spleen, brain, heart and liver. Ref.6

Post-translational modification: Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.

Involvement in disease: Bartter syndrome 2 (BS2) [MIM:241200]: An autosomal recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 2 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11 Ref.12 Ref.13

Sequence similarities: Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ1 subfamily. [View classification]