Heat Shock Protein 60 Recombinant Protein | HSP60 recombinant protein

Heat Shock Protein 60, Recombinant, Human (HSP60, GroEL, Chaperonin 60, cpn60)

Cat. Num. AAA636026

• Gene Names: HSP60; heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60; HSP60-3B
• Applications: ELISA, Western Blot
• Purity: Highly Purified; 90% pure as determined by SDS-PAGE analysis.
• Synonyms: Heat Shock Protein 60; Recombinant; Human (HSP60; GroEL; Chaperonin 60; cpn60); HSP60 recombinant protein

• Purity/Purification: Highly Purified; 90% pure as determined by SDS-PAGE analysis.
• Form/Format: Supplied as a liquid in PBS pH 7.5, 10% glycerol, 200mM Imidazole.

• Applicable Applications for HSP60 recombinant protein: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ATPase Assays, Binding Assays, as a Western Blot control and as an ELISA reference standard..
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for HSP60 recombinant protein

In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60 (1-3). Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its cochaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of Hsp60 and Hsp10 is their protective functions against infection and cellular stress. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).

Product Categories/Family for HSP60 recombinant protein

Molecular Biology; MB-Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 15229559
• NCBI GeneID: 821983
• NCBI Accession #: NP_189041.1
• NCBI GenBank Nucleotide #: NM_113304.4
• UniProt Accession #: P29197; Q9LIQ8
• Molecular Weight: 61,281 Da
• NCBI Official Full Name: heat shock protein 60
• NCBI Official Symbol: HSP60
• NCBI Official Synonym Symbols: heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60; HSP60-3B
• NCBI Protein Information: heat shock protein 60
• UniProt Protein Name: Chaperonin CPN60, mitochondrial
• Protein Family: Heat shock protein
• UniProt Gene Name: CPN60
• UniProt Entry Name: CH60A_ARATH

NCBI Description

mitochondrial chaperonin HSP. assist in rapid assembly of the oligomeric protein structures in the mitochondria.

Uniprot Description

Function: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Subcellular location: Mitochondrion.

Induction: By heat shock.

Sequence similarities: Belongs to the chaperonin (HSP60) family.