SDS-Page
(SDS-PAGE analysis: Lane 1: MWM, Lane 2: 2.0ug of purified Mouse HSP60 Protein (H1830-81))
Heat Shock Protein 60 Recombinant Protein | HSP60 recombinant protein
Heat Shock Protein 60, Recombinant, Mouse (HSP60, GroEL, Chaperonin 60, cpn60)
Gene Names
HSP60; heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60; HSP60-3B
Applications
Western Blot
Purity
Highly Purified90% as assayed by SDS-PAGE.
Synonyms
Heat Shock Protein 60; Recombinant; Mouse (HSP60; GroEL; Chaperonin 60; cpn60); HSP60 recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
90% as assayed by SDS-PAGE.
90% as assayed by SDS-PAGE.
Form/Format
Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 150mM sodium chloride, 1mM EDTA, 1mM DTT.
Applicable Applications for HSP60 recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot analysis. We recommend that the material be diluted in SDS containing sample buffer (e.g. Laemmli, U.K., (1970) Nature 277: 680-685) and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a colorimetric Western Blot. This protein does not contain GroEL as determined by Western Blot analysis.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
SDS-Page
(SDS-PAGE analysis: Lane 1: MWM, Lane 2: 2.0ug of purified Mouse HSP60 Protein (H1830-81))
SDS-Page
(SDS-PAGE analysis: Lane 1: MWM, Lane 2: 2.0ug of purified Mouse HSP60 Protein (H1830-81))
Western Blot (WB)
(Western Blot analysis: Lane 1: MW marker; Lane 2: 100ng H1830-81; Lane 3: 100ng E. coli GroEL Protein; Left Blot probed with HSP60 mAb at 1ug/ml; Right Blot probed with GroEL mAb at 1ug/ml.)
Western Blot (WB)
(Western Blot analysis: Lane 1: MW marker; Lane 2: 100ng H1830-81; Lane 3: 100ng E. coli GroEL Protein; Left Blot probed with HSP60 mAb at 1ug/ml; Right Blot probed with GroEL mAb at 1ug/ml.)
Related Product Information for HSP60 recombinant protein
The mouse Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65kD major antigen of mycobacteria. In eukaryotes, Hsp60 is
Product Categories/Family for HSP60 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,281 Da
NCBI Official Full Name
heat shock protein 60
NCBI Official Symbol
HSP60
NCBI Official Synonym Symbols
heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60; HSP60-3B
NCBI Protein Information
heat shock protein 60
UniProt Protein Name
Chaperonin CPN60, mitochondrial
Protein Family
UniProt Gene Name
CPN60
UniProt Entry Name
CH60A_ARATH
NCBI Description
mitochondrial chaperonin HSP. assist in rapid assembly of the oligomeric protein structures in the mitochondria.
Uniprot Description
Function: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Subcellular location: Mitochondrion.
Induction: By heat shock.
Sequence similarities: Belongs to the chaperonin (HSP60) family.
Similar Products
Product Notes
The HSP60 cpn60 (Catalog #AAA636857) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heat Shock Protein 60 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot analysis. We recommend that the material be diluted in SDS containing sample buffer (e.g. Laemmli, U.K., (1970) Nature 277: 680-685) and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a colorimetric Western Blot. This protein does not contain GroEL as determined by Western Blot analysis. Researchers should empirically determine the suitability of the HSP60 cpn60 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 60, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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