Western Blot (WB)
Mouse anti-Human, mouse Heat shock protein 60, HSP60 Monoclonal Antibody | anti-HSP60 antibody
Anti-Heat shock protein 60, HSP60 antibody
Gene Names
HSP60; heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60; HSP60-3B
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Protein A or Protein G purified
Synonyms
Heat shock protein 60; HSP60; Monoclonal Antibody; Anti-Heat shock protein 60; HSP60 antibody; Monoclonal Anti- Heat shock protein 60; anti-HSP60 antibody
Host
Mouse
Reactivity
Human, mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
H6-2
Purity/Purification
Protein A or Protein G purified
Form/Format
Liquid
Applicable Applications for anti-HSP60 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes
WB (1:5000)
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of HSP60.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-HSP60 antibody
Heat shock protein 60 (HSP60) in the matrix of mitochondria is essential for the folding and assembly of newly imported proteins. Hsp60 belongs to a class of structurally related chaperonins found in organelles of endosymbiotic origin and in the bacterial cytosol.1 Members of the chaperonin class include the Escherichia coli, groEL protein and the Rubisco subunit-binding protein of chloroplasts.2 In both prokaryotic and eukaryotic systems, synthesis of these proteins is induced in response to stresses, such as heat shock.3 Under normal physiological conditions, HSP60 is a 60 KD oligomer composed of monomers that form a complex arranged as two stacked heptameric rings.1 HSP60 may be a very useful marker for patients with superficial bladder carcinoma and may be used for predicting disease progression. And low HSP60 expression levels may be usable as a prognostic marker to identify patients for whom local treatment would be insufficient.4 HSP60 also linked to diabetes, cancer and certain types of immunological disorders.
References
1. Cheng MY, Hartl FU, Horwich AL (November 1990). "The mitochondrial chaperonin hsp60 is required for its own assembly". Nature 348 (6300): 455-8. 2. Cheng, M. Y., Hartl, F.-U., Martin, J., Pollock, R. A., Kalousek, F., Neupert, W., Hallberg, E. M., Hallberg, R. L., Horwich, A. L. Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989. 3. Venner, T. J., Singh, B., Gupta, R. S. Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families. DNA Cell Biol. 9: 545-552, 1990. 4. Lebret T, Watson RW, Molinié V, et al. (September 2003). "Heat shock proteins HSP27, HSP60, HSP70, and HSP90: expression in bladder carcinoma". Cancer 98 (5): 970-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,281 Da
NCBI Official Full Name
heat shock protein 60
NCBI Official Symbol
HSP60
NCBI Official Synonym Symbols
heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60; HSP60-3B
NCBI Protein Information
heat shock protein 60
UniProt Protein Name
Chaperonin CPN60, mitochondrial
Protein Family
UniProt Gene Name
CPN60
UniProt Entry Name
CH60A_ARATH
NCBI Description
mitochondrial chaperonin HSP. assist in rapid assembly of the oligomeric protein structures in the mitochondria.
Uniprot Description
Function: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Subcellular location: Mitochondrion.
Induction: By heat shock.
Sequence similarities: Belongs to the chaperonin (HSP60) family.
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Product Notes
The HSP60 cpn60 (Catalog #AAA355099) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Heat shock protein 60, HSP60 antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Heat shock protein 60, HSP60 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). WB (1:5000). Researchers should empirically determine the suitability of the HSP60 cpn60 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat shock protein 60, HSP60, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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