Fish Heat Shock Protein 60 (HSP-60) ELISA Kit | HSP-60 elisa kit

Fish Heat Shock Protein 60 (HSP-60) ELISA Kit

Cat. Num. AAA1601708

• Gene Names: HSP60; heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60-3B
• Reactivity: Fish
• Synonyms: Heat Shock Protein 60 (HSP-60); Fish Heat Shock Protein 60 (HSP-60) ELISA Kit; HSP-60 elisa kit

• Reactivity: Fish
• Sequence Length: 577

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.2ng/ml - 60ng/ml
• Sensitivity: 0.09ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSP-60 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Fish Heat Shock Protein 60 (also known as HSP-60) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Fish HSP-60 antibody. HSP-60 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Fish HSP-60 Antibody is added and binds to HSP-60 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSP-60 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Fish HSP-60. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 15229559
• NCBI GeneID: 821983
• NCBI Accession #: NP_189041.1
• NCBI GenBank Nucleotide #: NM_113304.5
• UniProt Accession #: P29197; Q9LIQ8
• Molecular Weight: 61,281 Da
• NCBI Official Full Name: heat shock protein 60
• NCBI Official Symbol: HSP60
• NCBI Official Synonym Symbols: heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60-3B
• NCBI Protein Information: heat shock protein 60
• UniProt Protein Name: Chaperonin CPN60, mitochondrial
• Protein Family: Chaperonin
• UniProt Gene Name: CPN60

NCBI Description

mitochondrial chaperonin HSP. assist in rapid assembly of the oligomeric protein structures in the mitochondria.

Uniprot Description

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.