UMPS recombinant protein

UMPS, 1-480aa, Human, His-tag, Baculovirus

Cat. Num. AAA206709

• Gene Names: UMPS; OPRT
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: UMPS; 1-480aa; Human; His-tag; Baculovirus; Uridine 5'-monophosphate synthase; OPRT; UMPS recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4).
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MAVARAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADILFQT AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL IIEDVVTSGS SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML EILEQQKKVD AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFGAR AELPRIHPVA SKLLRLMQKK ETNLCLSADV SLARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT LAKCHEFLIF EDRKFADIGN TVKKQYEGGI FKIASWADLV NAHVVPGSGV VKGLQEVGLP LHRGCLLIAE MSSTGSLATG DYTRAAVRMA EEHSEFVVGF ISGSRVSMKP EFLHLTPGVQ LEAGGDNLGQ QYNSPQEVIG KRGSDIIIVG RGIISAADRL EAAEMYRKAA WEAYLSRLGV HHHHHH
• Sequence Length: 480

• Applicable Applications for UMPS recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for UMPS recombinant protein

UMPS, also known as uridine 5'-monophosphate synthase, is a bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis. It is an indispensable component in this metabolic pathway and is a target for antimalarial and antitumor drugs. Its expression in colorectal carcinoma tissues is not correlated with the toxicities of 5-FU-based regimen, but It in the normal tissues can help predict the toxicities associated with 5-FU. Recombinant human UMPS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for UMPS recombinant protein

Epigenomics (Transcription & Translation)

References

Zhang Y., et al. (2013) J Biol Chem. 288:34746-34754.; Dong Q., et al. (2012) Nan Fang Yi Ke Da Xue Xue Bao. 32:1179-1181.

NCBI and Uniprot Product Information

• NCBI GI #: 4507835
• NCBI GeneID: 7372
• NCBI Accession #: NP_000364
• NCBI GenBank Nucleotide #: NM_000373.3
• UniProt Accession #: P11172; O00758; O00759; O00760; Q16862; Q9H3Q2; Q9UG49; B5LY68; B5LY72
• Molecular Weight: 53kDa (486aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: uridine 5'-monophosphate synthase
• NCBI Official Synonym Full Names: uridine monophosphate synthetase
• NCBI Official Symbol: UMPS
• NCBI Official Synonym Symbols: OPRT
• NCBI Protein Information: uridine 5'-monophosphate synthase
• UniProt Protein Name: Uridine 5'-monophosphate synthase
• Protein Family: Uridine 5'-monophosphate synthase
• UniProt Gene Name: UMPS
• UniProt Synonym Gene Names: UMP synthase; OPRT; OPRTase; ODC

NCBI Description

This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]