IDE Antibody | anti-IDE antibody

Goat anti-IDE (long isoform aa10-14) Antibody

Cat. Num. AAA423763

• Gene Names: IDE; INSULYSIN
• Reactivity: Human; Expected from sequence similarity: Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: IDE; Goat anti-IDE (long isoform aa10-14) Antibody; Goat Anti-Insulysin/Insulinase; Biotinylated Antibody; insulin-degrading enzyme; INSULYSIN; Abeta-degrading protease; insulin protease; insulinase; anti-IDE antibody

• Reactivity: Human; Expected from sequence similarity: Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 1019

• Applicable Applications for anti-IDE antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:2000.; WB: Approx 58-60kDa band observed in cell lysates of HEK293 and HeLa. Recommended concentration: 1-2ug/ml.
• Immunogen Sequence: Peptide with sequence HPALPSTFRSVLGAR-C, from the N Terminus of the protein sequence according to NP_004960.2; NP_001159418.1.
• Peptide Sequence + C: HPALPSTFRSVLGAR-C
• Epitope: N Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((1ug/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 155969707
• NCBI GeneID: 3416
• NCBI Accession #: NP_004960.2; NP_001159418.1
• NCBI GenBank Nucleotide #: NM_004969.3
• UniProt Accession #: P14735
• Molecular Weight: 54.2kDa
• NCBI Official Full Name: insulin-degrading enzyme isoform 1
• NCBI Official Synonym Full Names: insulin degrading enzyme
• NCBI Official Symbol: IDE
• NCBI Official Synonym Symbols: INSULYSIN
• NCBI Protein Information: insulin-degrading enzyme
• UniProt Protein Name: Insulin-degrading enzyme
• Protein Family: IgM protease
• UniProt Gene Name: IDE
• UniProt Synonym Gene Names: Insulinase

NCBI Description

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]

Uniprot Description

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.