Typical Testing Data/Standard Curve (for reference only)
Guinea Pig IDE ELISA Kit | IDE elisa kit
Guinea pig IDE (Insulin Degrading Enzyme) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IDE. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IDE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IDE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IDE. You can calculate the concentration of IDE in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]
Uniprot Description
IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; Cell surface; Nuclear receptor co-regulator; EC 3.4.24.56
Chromosomal Location of Human Ortholog: 10q23-q25
Cellular Component: nucleoplasm; extracellular space; peroxisomal matrix; cell surface; mitochondrion; cytoplasm; plasma membrane; peroxisome; nucleus; cytosol
Molecular Function: ubiquitin binding; protein homodimerization activity; zinc ion binding; metalloendopeptidase activity; beta-amyloid binding; ATPase activity; peptide binding; beta-endorphin binding; insulin binding; protein binding; glycoprotein binding; ATP binding; receptor binding
Biological Process: negative regulation of proteolysis; positive regulation of protein oligomerization; viral reproduction; proteolysis involved in cellular protein catabolic process; protein heterooligomerization; beta-amyloid metabolic process; insulin receptor signaling pathway; hormone catabolic process; determination of adult life span; proteolysis; protein homooligomerization; protein homotetramerization
Research Articles on IDE
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Product Notes
The Guinea Pig IDE ide (Catalog #AAA2513196) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2513196 ELISA Kit recognizes Guinea Pig IDE. It is sometimes possible for the material contained within the vial of "IDE, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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